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The multifunctional enzyme, type‐1 (MFE1) is involved in several lipid metabolizing pathways. It catalyses: (a) enoyl‐CoA isomerase and (b) enoyl‐CoA hydratase (EC 4.2.1.17) reactions in its N‐terminal crotonase part, as well as (3) a 3S‐hydroxy‐acyl‐CoA dehydrogenase (HAD; EC 1.1.1.35) reaction in its C‐terminal 3S‐hydroxy‐acyl‐CoA dehydrogenase part. Crystallographic binding studies with rat peroxisomal...
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