Search results for: Janet M. Wood

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article

Cardiolipin synthase A colocalizes with cardiolipin and osmosensing transporter ProP at the poles of Escherichia coli cells

Tatyana Romantsov, Karen Gonzalez, Naheda Sahtout, Doreen E. Culham, more

Molecular Microbiology > 107 > 5 > 623 - 638

Osmosensing by transporter ProP is modulated by its cardiolipin (CL)‐dependent concentration at the poles of Escherichia coli cells. Other contributors to this phenomenon were sought with the BACterial Two‐Hybrid System (BACTH). The BACTH‐tagged variants T18‐ProP and T25‐ProP retained ProP function and localization. Their interaction confirmed the ProP homo‐dimerization previously established by protein...

article

Perspective: challenges and opportunities for the study of cardiolipin, a key player in bacterial cell structure and function

Janet M. Wood

Current Genetics > 2018 > 64 > 4 > 795-798

Cardiolipin (CL) is a key player in bacterial cell biology. CL accumulates at the poles of rod-shaped cells; the polar localization and function of diverse bacterial proteins are CL-dependent. Cardiolipin (CL) is an unusual phospholipid comprised of a glycerol headgroup coupled with two phosphatidate moieties. CL-rich membrane domains are often visualized with the fluorescent indicator 10-N-nonyl-acridine...

article

ProP‐ProP and ProP‐phospholipid interactions determine the subcellular distribution of osmosensing transporter ProP in Escherichia coli

Tatyana Romantsov, Doreen E. Culham, Tavia Caplan, Jennifer Garner, more

Molecular Microbiology > 103 > 3 > 469 - 482

Osmosensing transporter ProP protects bacteria from osmotically induced dehydration by mediating the uptake of zwitterionic osmolytes. ProP activity is a sigmoidal function of the osmolality. ProP orthologues share an extended, cytoplasmic C‐terminal domain. Orthologues with and without a C‐terminal, α‐helical coiled‐coil domain respond similarly to the osmolality. ProP concentrates at the poles and...

article

ProQ Is an RNA Chaperone that Controls ProP Levels in Escherichia coli

Steven G. Chaulk, Michelle N. Smith−Frieday, David C. Arthur, Doreen E. Culham, more

Biochemistry > 2011 > 50 > 15 > 3095 - 3106

article

Transmembrane Helix I and Periplasmic Loop 1 of Escherichia coli ProP Are Involved in Osmosensing and Osmoprotectant Transport

Robert A. B. Keates, Doreen E. Culham, Yaroslava I. Vernikovska, Adam J. Zuiani, more

Biochemistry > 2010 > 49 > 41 > 8847 - 8856

article

Cardiolipin and the osmotic stress responses of bacteria

Tatyana Romantsov, Ziqiang Guan, Janet M. Wood

BBA - Biomembranes > 2009 > 1788 > 10 > 2092-2100

Cells control their own hydration by accumulating solutes when they are exposed to high osmolality media and releasing solutes in response to osmotic down-shocks. Osmosensory transporters mediate solute accumulation and mechanosensitive channels mediate solute release. Escherichia coli serves as a paradigm for studies of cellular osmoregulation. Growth in media of high salinity alters the phospholipid...

article

Roles of K ⁺ , H ⁺ , H ₂ O, and ΔΨ in Solute Transport Mediated by Major Facilitator Superfamily Members ProP and LacY ^†

Doreen E. Culham, Tatyana Romantsov, Janet M. Wood

Biochemistry > 2008 > 47 > 31 > 8176 - 8185

article

Core Residue Replacements Cause Coiled-Coil Orientation Switching in Vitro and in Vivo : Structure−Function Correlations for Osmosensory Transporter ProP ^†

Yonit Tsatskis, Stanley C. Kwok, Elisabeth Becker, Chad Gill, more

Biochemistry > 2008 > 47 > 1 > 60 - 72

article

Structure and Function of Transmembrane Segment XII in Osmosensor and Osmoprotectant Transporter ProP of Escherichia coli ^†

Feng Liu, Doreen E. Culham, Yaroslava I. Vernikovska, Robert A. B. Keates, more

Biochemistry > 2007 > 46 > 19 > 5647 - 5655

article

Structural and Functional Analysis of ProQ: An Osmoregulatory Protein of Escherichia coli ^†

Michelle N. Smith, Stanley C. Kwok, Robert S. Hodges, Janet M. Wood

Biochemistry > 2007 > 46 > 11 > 3084 - 3095

article

Formation of an Antiparallel, Intermolecular Coiled Coil Is Associated with in Vivo Dimerization of Osmosensor and Osmoprotectant Transporter ProP in Escherichia coli

Alexander Hillar, Doreen E. Culham, Yaroslava I. Vernikovska, Janet M. Wood, more

Biochemistry > 2005 > 44 > 47 > 15636 - 15636

article

Formation of an Antiparallel, Intermolecular Coiled Coil Is Associated with in Vivo Dimerization of Osmosensor and Osmoprotectant Transporter ProP in Escherichia coli ^†

Alexander Hillar, Doreen E. Culham, Yaroslava I. Vernikovska, Janet M. Wood, more

Biochemistry > 2005 > 44 > 30 > 10170 - 10180

article

A Structural Model for the Osmosensor, Transporter, and Osmoregulator ProP of Escherichia coli ^†

Janet M. Wood, Doreen E. Culham, Alexander Hillar, Yaroslava I. Vernikovska, more

Biochemistry > 2005 > 44 > 15 > 5634 - 5646

article

Bacterial osmosensing: roles of membrane structure and electrostatics in lipid–protein and protein–protein interactions

Bert Poolman, Jan J. Spitzer, Janet M. Wood

BBA - Biomembranes > 2004 > 1666 > 1-2 > 88-104

Bacteria act to maintain their hydration when the osmotic pressure of their environment changes. When the external osmolality decreases (osmotic downshift), mechanosensitive channels are activated to release low molecular weight osmolytes (and hence water) from the cytoplasm. Upon osmotic upshift, osmoregulatory transporters are activated to import osmolytes (and hence water). Osmoregulatory channels...

article

Overexpression, Purification, and Characterization of ProQ, a Posttranslational Regulator for Osmoregulatory Transporter ProP of Escherichia coli ^†

Michelle N. Smith, Rebecca A. Crane, Robert A. B. Keates, Janet M. Wood

Biochemistry > 2004 > 43 > 41 > 12979 - 12989

article

Solution Structure of the C-terminal Antiparallel Coiled-coil Domain from Escherichia coli Osmosensor ProP

David L Zoetewey, Brian P Tripet, Tatiana G Kutateladze, Michael J Overduin, more

Journal of Molecular Biology > 2003 > 334 > 5 > 1063-1076

Bacteria respond to increasing medium osmolality by accumulating organic solutes that are compatible with cellular functions. Transporter ProP of Escherichia coli, a proton symporter and a member of the major facilitator superfamily, senses osmotic shifts and responds by importing osmolytes such as glycine betaine. ProP contains a cytoplasmic, C-terminal extension that is essential for its activity...

article

Detection of α-Helical Coiled-Coil Dimer Formation by Spin-Labeled Synthetic Peptides: A Model Parallel Coiled-Coil Peptide and the Antiparallel Coiled Coil Formed by a Replica of the ProP C-Terminus ^†

Alexander Hillar, Brian Tripet, David Zoetewey, Janet M. Wood, more

Biochemistry > 2003 > 42 > 51 > 15170 - 15178

article

Creation of a Fully Functional Cysteine-Less Variant of Osmosensor and Proton-Osmoprotectant Symporter ProP from Escherichia coli and Its Application to Assess the Transporter's Membrane Orientation ^†

Doreen E. Culham, Alexander Hillar, James Henderson, Anh Ly, more

Biochemistry > 2003 > 42 > 40 > 11815 - 11823

article

Osmosensor ProP of Escherichia coli Responds to the Concentration, Chemistry, and Molecular Size of Osmolytes in the Proteoliposome Lumen ^† ^, ^‡

Doreen E. Culham, James Henderson, Rebecca A. Crane, Janet M. Wood

Biochemistry > 2003 > 42 > 2 > 410 - 420

article

Osmosensing and osmoregulatory compatible solute accumulation by bacteria

Janet M. Wood, Erhard Bremer, Laszlo N. Csonka, Reinhard Kraemer, more

Comparative Biochemistry and Physiology, Part A > 2001 > 130 > 3 > 437-460

Bacteria inhabit natural and artificial environments with diverse and fluctuating osmolalities, salinities and temperatures. Many maintain cytoplasmic hydration, growth and survival most effectively by accumulating kosmotropic organic solutes (compatible solutes) when medium osmolality is high or temperature is low (above freezing). They release these solutes into their environment when the medium...

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Search results for: Janet M. Wood

Cardiolipin synthase A colocalizes with cardiolipin and osmosensing transporter ProP at the poles of Escherichia coli cells

Perspective: challenges and opportunities for the study of cardiolipin, a key player in bacterial cell structure and function

ProP‐ProP and ProP‐phospholipid interactions determine the subcellular distribution of osmosensing transporter ProP in Escherichia coli

ProQ Is an RNA Chaperone that Controls ProP Levels in Escherichia coli

Transmembrane Helix I and Periplasmic Loop 1 of Escherichia coli ProP Are Involved in Osmosensing and Osmoprotectant Transport

Cardiolipin and the osmotic stress responses of bacteria

Roles of K ⁺ , H ⁺ , H ₂ O, and ΔΨ in Solute Transport Mediated by Major Facilitator Superfamily Members ProP and LacY ^†

Core Residue Replacements Cause Coiled-Coil Orientation Switching in Vitro and in Vivo : Structure−Function Correlations for Osmosensory Transporter ProP ^†

Structure and Function of Transmembrane Segment XII in Osmosensor and Osmoprotectant Transporter ProP of Escherichia coli ^†

Structural and Functional Analysis of ProQ: An Osmoregulatory Protein of Escherichia coli ^†

Formation of an Antiparallel, Intermolecular Coiled Coil Is Associated with in Vivo Dimerization of Osmosensor and Osmoprotectant Transporter ProP in Escherichia coli

Formation of an Antiparallel, Intermolecular Coiled Coil Is Associated with in Vivo Dimerization of Osmosensor and Osmoprotectant Transporter ProP in Escherichia coli ^†

A Structural Model for the Osmosensor, Transporter, and Osmoregulator ProP of Escherichia coli ^†

Bacterial osmosensing: roles of membrane structure and electrostatics in lipid–protein and protein–protein interactions

Overexpression, Purification, and Characterization of ProQ, a Posttranslational Regulator for Osmoregulatory Transporter ProP of Escherichia coli ^†

Solution Structure of the C-terminal Antiparallel Coiled-coil Domain from Escherichia coli Osmosensor ProP

Detection of α-Helical Coiled-Coil Dimer Formation by Spin-Labeled Synthetic Peptides: A Model Parallel Coiled-Coil Peptide and the Antiparallel Coiled Coil Formed by a Replica of the ProP C-Terminus ^†

Creation of a Fully Functional Cysteine-Less Variant of Osmosensor and Proton-Osmoprotectant Symporter ProP from Escherichia coli and Its Application to Assess the Transporter's Membrane Orientation ^†

Osmosensor ProP of Escherichia coli Responds to the Concentration, Chemistry, and Molecular Size of Osmolytes in the Proteoliposome Lumen ^† ^, ^‡

Osmosensing and osmoregulatory compatible solute accumulation by bacteria

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