Search results for: Albert H. Beth

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chapter

Saturation Transfer EPR

Albert H. Beth, Eric J. Hustedt

Biological Magnetic Resonance > Biomedical EPR, Part B: Methodology, Instrumentation, and Dynamics > Motion, Proteins, and Membranes > 369-407

Saturation transfer EPR (ST-EPR) has been utilized to characterize the very slow rotational diffusion of a wide range of membrane proteins over the past three decades. Development of computational algorithms for analyzing the ST-EPR data from membrane proteins undergoinguniaxial rotational diffusion and inclusion of these algorithms into global nonlinear least squares fitting routines has permitted...

article

CLC Anion Channel Regulatory Phosphorylation and Conserved Signal Transduction Domains

Hiroaki Miyazaki, Toshiki Yamada, Angela Parton, Rebecca Morrison, more

Biophysical Journal > 2012 > 103 > 8 > 1706-1718

The signaling mechanisms that regulate CLC anion channels are poorly understood. Caenorhabditis elegans CLH-3b is a member of the CLC-1/2/Ka/Kb channel subfamily. CLH-3b is activated by meiotic cell-cycle progression and cell swelling. Inhibition is brought about by GCK-3 kinase-mediated phosphorylation of S742 and S747 located on a ∼176 amino acid disordered domain linking CBS1 and CBS2. Much of...

article

Rotational Diffusion of Membrane Proteins: Characterization of Protein-Protein Interactions in Membranes

Albert H. Beth

Biophysical Journal > 2012 > 103 > 6 > 1109

article

The global analysis of DEER data

Suzanne Brandon, Albert H. Beth, Eric J. Hustedt

Journal of Magnetic Resonance > 2012 > 218 > Complete > 93-104

Double Electron–Electron Resonance (DEER) has emerged as a powerful technique for measuring long range distances and distance distributions between paramagnetic centers in biomolecules. This information can then be used to characterize functionally relevant structural and dynamic properties of biological molecules and their macromolecular assemblies. Approaches have been developed for analyzing experimental...

article

Structure of the Intracellular Ca2+-Binding Domain of NCX1.1 by Site Directed Spin Labeling

Neena Dixit, Sunghoon Kim, Gage Matthews, Eric J. Hustedt, more

Biophysical Journal > 2011 > 100 > 3 Supplement 1 > 146a

article

Site-Directed Spin-Labeling Studies: Structure of the AnkD34-Cdb3 Complex

Sunghoon Kim, Suzanne Brandon, Jarrod A. Smith, Sarah J. Edwards, more

Biophysical Journal > 2011 > 100 > 3 Supplement 1 > 145a

article

Structure and Dynamics of the Calcium Binding Domains of the Na/Ca Exchanger (NCX1.1) Determined by Site Directed Spin Labeling

Mrinalini Dixit, Sunghoon Kim, Eric J. Hustedt, Charles E. Cobb, more

Biophysical Journal > 2010 > 98 > 3 Supplement 1 > 399a-400a

article

Structure of the CDB3 - ankD34 Complex from Site - Directed Spin - Labeling Studies

Sunghoon Kim, Eric J. Hustedt, Suzanne Brandon, Charles E. Cobb, more

Biophysical Journal > 2010 > 98 > 3 Supplement 1 > 400a

article

Structure of the cdb3-ankD34 Complex from Site Directed Spin Labeling Studies

Sunghoon Kim, Eric J. Hustedt, Suzanne Brandon, Charles E. Cobb, more

Biophysical Journal > 2009 > 96 > 3 Supplement 1 > 309a-310a

article

Simulation of Nitroxide Electron Paramagnetic Resonance Spectra from Brownian Trajectories and Molecular Dynamics Simulations

Susan C. DeSensi, David P. Rangel, Albert H. Beth, Terry P. Lybrand, more

Biophysical Journal > 2008 > 94 > 10 > 3798-3809

A simulated continuous wave electron paramagnetic resonance spectrum of a nitroxide spin label can be obtained from the Fourier transform of a free induction decay. It has been previously shown that the free induction decay can be calculated by solving the time-dependent stochastic Liouville equation for a set of Brownian trajectories defining the rotational dynamics of the label. In this work, a...

article

How to Assemble the Parts: Structures of Protein Complexes from their Components

Albert H. Beth

Biophysical Journal > 2007 > 93 > 10 > 3339

article

Structure of the Cytoplasmic Domain of Erythrocyte Band 3 Hereditary Spherocytosis Variant P327R: Band 3 Tuscaloosa ^†

Zheng Zhou, Susan C. DeSensi, Richard A. Stein, Suzanne Brandon, more

Biochemistry > 2007 > 46 > 36 > 10248 - 10257

article

Solution Structure of the Cytoplasmic Domain of Erythrocyte Membrane Band 3 Determined by Site-Directed Spin Labeling ^†

Zheng Zhou, Susan C. DeSensi, Richard A. Stein, Suzanne Brandon, more

Biochemistry > 2005 > 44 > 46 > 15115 - 15128

article

Functional Effects of Glycosylation at Asn-579 of the Epidermal Growth Factor Receptor ^†

Kristin B. Whitson, Stefanie R. Whitson, Monica L. Red-Brewer, Austin J. McCoy, more

Biochemistry > 2005 > 44 > 45 > 14920 - 14931

article

Formulation of Zeeman modulation as a signal filter

Robert D. Nielsen, Eric J. Hustedt, Albert H. Beth, Bruce H. Robinson

Journal of Magnetic Resonance > 2004 > 170 > 2 > 345-371

The Bloch equation containing a Zeeman modulation field is solved analytically by treating the Zeeman modulation frequency as a perturbation. The absorption and dispersion signals at both 0° and 90° modulation phase are obtained. The solutions are valid to first order in the modulation frequency, but are otherwise valid for any value of modulation amplitude or microwave amplitude. A first order treatment...

article

High Field/High Frequency Saturation Transfer Electron Paramagnetic Resonance Spectroscopy: Increased Sensitivity to Very Slow Rotational Motions

Eric J. Hustedt, Albert H. Beth

Biophysical Journal > 2004 > 86 > 6 > 3940-3950

Saturation transfer electron paramagnetic resonance (ST-EPR) spectroscopy has been employed to characterize the very slow microsecond to millisecond rotational dynamics of a wide range of nitroxide spin-labeled proteins and other macromolecules in the past three decades. The vast majority of this previous work has been carried out on spectrometers that operate at X-band (∼9GHz) microwave frequency...

article

Preparation and characterization of Alexa Fluor 594-labeled epidermal growth factor for fluorescence resonance energy transfer studies: application to the epidermal growth factor receptor

Kristin B. Whitson, Joseph M. Beechem, Albert H. Beth, James V. Staros

Analytical Biochemistry > 2004 > 324 > 2 > 227-236

We have prepared and characterized a new fluorescent derivative of murine epidermal growth factor (EGF), Alexa Fluor 594-labeled EGF (A-EGF), for fluorescence studies of EGF-EGF receptor interactions. We describe the synthesis of this derivative and its physical and biological characterization. The significant overlap between the excitation and the emission spectra of A-EGF makes this probe well suited...

article

Rotational Dynamics of the Epidermal Growth Factor Receptor ^†

Richard A. Stein, Eric J. Hustedt, James V. Staros, Albert H. Beth

Biochemistry > 2002 > 41 > 6 > 1957 - 1964

article

The Sensitivity of Saturation Transfer Electron Paramagnetic Resonance Spectra to Restricted Amplitude Uniaxial Rotational Diffusion

Eric J. Hustedt, Albert H. Beth

Biophysical Journal > 2001 > 81 > 6 > 3156-3165

Computational methods have been developed to model the effects of constrained or restricted amplitude uniaxial rotational diffusion (URD) on saturation transfer electron paramagnetic resonance (ST-EPR) signals observed from nitroxide spin labels. These methods, which have been developed to model the global rotational motion of intrinsic membrane proteins that can interact with the cytoskeleton or...

article

Flexibility of the Cytoplasmic Domain of the Anion Exchange Protein, Band 3, in Human Erythrocytes

Scott M. Blackman, Eric J. Hustedt, Charles E. Cobb, Albert H. Beth

Biophysical Journal > 2001 > 81 > 6 > 3363-3376

The rotational flexibility of the cytoplasmic domain of band 3, in the region that is proximal to the inner membrane surface, has been investigated using a combination of time-resolved optical anisotropy (TOA) and saturation-transfer electron paramagnetic resonance (ST-EPR) spectroscopies. TOA studies of rotational diffusion of the transmembrane domain of band 3 show a dramatic decrease in residual...

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Search results for: Albert H. Beth

Saturation Transfer EPR

CLC Anion Channel Regulatory Phosphorylation and Conserved Signal Transduction Domains

Rotational Diffusion of Membrane Proteins: Characterization of Protein-Protein Interactions in Membranes

The global analysis of DEER data

Structure of the Intracellular Ca2+-Binding Domain of NCX1.1 by Site Directed Spin Labeling

Site-Directed Spin-Labeling Studies: Structure of the AnkD34-Cdb3 Complex

Structure and Dynamics of the Calcium Binding Domains of the Na/Ca Exchanger (NCX1.1) Determined by Site Directed Spin Labeling

Structure of the CDB3 - ankD34 Complex from Site - Directed Spin - Labeling Studies

Structure of the cdb3-ankD34 Complex from Site Directed Spin Labeling Studies

Simulation of Nitroxide Electron Paramagnetic Resonance Spectra from Brownian Trajectories and Molecular Dynamics Simulations

How to Assemble the Parts: Structures of Protein Complexes from their Components

Structure of the Cytoplasmic Domain of Erythrocyte Band 3 Hereditary Spherocytosis Variant P327R: Band 3 Tuscaloosa ^†

Solution Structure of the Cytoplasmic Domain of Erythrocyte Membrane Band 3 Determined by Site-Directed Spin Labeling ^†

Functional Effects of Glycosylation at Asn-579 of the Epidermal Growth Factor Receptor ^†

Formulation of Zeeman modulation as a signal filter

High Field/High Frequency Saturation Transfer Electron Paramagnetic Resonance Spectroscopy: Increased Sensitivity to Very Slow Rotational Motions

Preparation and characterization of Alexa Fluor 594-labeled epidermal growth factor for fluorescence resonance energy transfer studies: application to the epidermal growth factor receptor

Rotational Dynamics of the Epidermal Growth Factor Receptor ^†

The Sensitivity of Saturation Transfer Electron Paramagnetic Resonance Spectra to Restricted Amplitude Uniaxial Rotational Diffusion

Flexibility of the Cytoplasmic Domain of the Anion Exchange Protein, Band 3, in Human Erythrocytes

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