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One of the major allergens from the pollen of perennial rye grass (Lolium perenne), Lol pII, was used to isolate specific antibody fragments from a random combinatorial library displaying a large repertoire of human Fab on filamentous phages. After five panning cycles on recombinantLol p II immunotubes, phage binders were isolated and the antibody fragments expressed as soluble Fab molecules in the...
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