Search results for: René Buchet

Items from 1 to 20 out of 49 results

article

Three‐dimensional cell‐laden collagen scaffolds: From biochemistry to bone bioengineering

Lucas Fabricio Bahia Nogueira, Bianca C. Maniglia, Rene Buchet, José Luis Millán, more

Journal of Biomedical Materials Research Part B: Applied Biomaterials > 110 > 4 > 967 - 983

The bones can be viewed as both an organ and a material. As an organ, the bones give structure to the body, facilitate skeletal movement, and provide protection to internal organs. As a material, the bones consist of a hybrid organic/inorganic three‐dimensional (3D) matrix, composed mainly of collagen, noncollagenous proteins, and a calcium phosphate mineral phase, which is formed and regulated by...

article

Effects of phospholipase D during cultured osteoblast mineralization and bone formation

Dina Abdallah, Najwa Skafi, Eva Hamade, Mathieu Borel, more

Journal of Cellular Biochemistry > 120 > 4 > 5923 - 5935

Mammalian phospholipase D (PLD) mostly hydrolyzes phosphatidylcholine producing phosphatidic acid. PLD activity was previously detected in different osteoblastic cell models, and was increased by several growth factors involved in bone homeostasis. To confirm possible actions of PLD isoforms during mineralization process, we analyzed their effects in osteoblastic cell models and during bone formation...

article

Phospholipase D: A new mediator during high phosphate‐induced vascular calcification associated with chronic kidney disease

Najwa Skafi, Dina Abdallah, Christophe Soulage, Sophie Reibel, more

Journal of Cellular Physiology > 234 > 4 > 4825 - 4839

Vascular calcification (VC) is the pathological accumulation of calcium phosphate crystals in one of the layers of blood vessels, leading to loss of elasticity and causing severe calcification in vessels. Medial calcification is mostly seen in patients with chronic kidney disease (CKD) and diabetes. Identification of key enzymes and their actions during calcification will contribute to understand...

article

Lipid microenvironment affects the ability of proteoliposomes harboring TNAP to induce mineralization without nucleators

Ana Maria Sper Simão, Maytê Bolean, Bruno Zoccaratto Favarin, Ekeveliny Amabile Veschi, more

Journal of Bone and Mineral Metabolism > 2019 > 37 > 4 > 607-613

Tissue-nonspecific alkaline phosphatase (TNAP), a glycosylphosphatidylinositol-anchored ectoenzyme present on the membrane of matrix vesicles (MVs), hydrolyzes the mineralization inhibitor inorganic pyrophosphate as well as ATP to generate the inorganic phosphate needed for apatite formation. Herein, we used proteoliposomes harboring TNAP as MV biomimetics with or without nucleators of mineral formation...

article

Collagen promotes matrix vesicle-mediated mineralization by vascular smooth muscle cells

Monika Roszkowska, Agnieszka Strzelecka-Kiliszek, Laurence Bessueille, René Buchet, more

Journal of Inorganic Biochemistry > 2018 > 186 > C > 1-9

Vascular calcification (VC) is a hallmark of atherosclerotic plaques. Calcification of advanced plaques shares common features with endochondral ossification of long bones and appears to be protective. On the other hand, microcalcification of early plaques, which is poorly understood, is thought to be harmful. Tissue-nonspecific alkaline phosphatase (TNAP) and collagen are the two proteins necessary...

article

Matrix vesicles from chondrocytes and osteoblasts: Their biogenesis, properties, functions and biomimetic models

Massimo Bottini, Saida Mebarek, Karen L. Anderson, Agnieszka Strzelecka-Kiliszek, more

Biochimica et Biophysica Acta (BBA) - General Subjects > 2018 > 1862 > 3 > 532-546

Matrix vesicles (MVs) are released from hypertrophic chondrocytes and from mature osteoblasts, the cells responsible for endochondral and membranous ossification. Under pathological conditions, they can also be released from cells of non-skeletal tissues such as vascular smooth muscle cells. MVs are extracellular vesicles of approximately 100–300nm diameter harboring the biochemical machinery needed...

article

Characteristics of minerals in vesicles produced by human osteoblasts hFOB 1.19 and osteosarcoma Saos-2 cells stimulated for mineralization

Agnieszka Strzelecka-Kiliszek, Lukasz Bozycki, Saida Mebarek, Rene Buchet, more

Journal of Inorganic Biochemistry > 2017 > 171 > C > 100-107

Bone cells control initial steps of mineralization by producing extracellular matrix (ECM) proteins and releasing vesicles that trigger apatite nucleation. Using transmission electron microscopy with energy dispersive X-ray microanalysis (TEM-EDX) we compared the quality of minerals in vesicles produced by two distinct human cell lines: fetal osteoblastic hFOB 1.19 and osteosarcoma Saos-2. Both cell...

article

Functions of Rho family of small GTPases and Rho-associated coiled-coil kinases in bone cells during differentiation and mineralization

Agnieszka Strzelecka-Kiliszek, Saida Mebarek, Monika Roszkowska, René Buchet, more

Biochimica et Biophysica Acta (BBA) - General Subjects > 2017 > 1861 > 5(PA) > 1009-1023

Members of Rho-associated coiled-coil kinases (ROCKs) are effectors of Rho family of small GTPases. ROCKs have multiple functions that include regulation of cellular contraction and polarity, adhesion, motility, proliferation, apoptosis, differentiation, maturation and remodeling of the extracellular matrix (ECM).Here, we focus on the action of RhoA and RhoA effectors, ROCK1 and ROCK2, in cells related...

article

TNAP stimulates vascular smooth muscle cell trans-differentiation into chondrocytes through calcium deposition and BMP-2 activation: Possible implication in atherosclerotic plaque stability

Maya Fakhry, Monika Roszkowska, Anne Briolay, Carole Bougault, more

Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease > 2017 > 1863 > 3 > 643-653

Atherosclerotic plaque calcification varies from early, diffuse microcalcifications to a bone-like tissue formed by endochondral ossification. Recently, a paradigm has emerged suggesting that if the bone metaplasia stabilizes the plaques, microcalcifications are harmful. Tissue-nonspecific alkaline phosphatase (TNAP), an ectoenzyme necessary for mineralization by its ability to hydrolyze inorganic...

article

Determination of phosphatase activity in osteoblasts by IR and two-dimensional correlation IR spectroscopy

Zhongyuan Ren, Liping Zhang, Yuqing Wu, Saida Mebarek, more

Vibrational Spectroscopy > 2016 > 86 > C > 206-211

Phosphatase activity in osteoblasts like Saos-2 cells was previously determined by measuring infrared spectra of substrates (pyrophosphate, 4-nitrophenyl phosphate, α-glucose-1-phosphate or β-Glycerophosphate), in the presence of Saos-2 cells under physiological conditions, at 37°C and pH=8. In this work, 2D correlation analysis served to resolve component bands in infrared spectra to obtain accurate...

article

Synthesis of benzofuran derivatives as selective inhibitors of tissue-nonspecific alkaline phosphatase: effects on cell toxicity and osteoblast-induced mineralization

Stéphanie Marquès, René Buchet, Florence Popowycz, Marc Lemaire, more

Bioorganic & Medicinal Chemistry Letters > 2016 > 26 > 5 > 1457-1459

Tissue-nonspecific alkaline phosphatase (TNAP) by hydrolyzing pyrophosphate, an inhibitor of apatite formation, promotes extracellular matrix calcification during bone formation and growth, as well as during ectopic calcification under pathological conditions. TNAP is a target for the treatment of soft tissue pathological ossification. We synthesized a series of benzofuran derivatives. Among these,...

article

Fatty acid composition in matrix vesicles and in microvilli from femurs of chicken embryos revealed selective recruitment of fatty acids

Dina Abdallah, Eva Hamade, Raghida Abou Merhi, Badran Bassam, more

Biochemical and Biophysical Research Communications > 2014 > 446 > 4 > 1161-1164

Hypertrophic chondrocytes participate in matrix mineralization by releasing matrix vesicles (MVs). These MVs, by accumulating Ca ²⁺ and phosphate initiate the formation of hydroxyapatite. To determine the types of lipids essential for mineralization, we analyzed fatty acids (FAs) in MVs, microvilli and in membrane fractions of chondrocytes isolated from femurs of chicken embryos. The FA composition...

article

Inhibitors of tissue-nonspecific alkaline phosphatase: Design, synthesis, kinetics, biomineralization and cellular tests

Julien Debray, Lei Chang, Stéphanie Marquès, Stéphane Pellet-Rostaing, more

Bioorganic & Medicinal Chemistry > 2013 > 21 > 24 > 7981-7987

Chronic kidney disease (CKD) is associated with numerous metabolic and endocrine disturbances, including abnormalities of calcium and phosphate metabolism and an inflammatory syndrome. The latter occurs early in the course of CKD and contributes to the development and progression of vascular calcification. A few therapeutic strategies are today contemplated to target vascular calcification in patients...

article

Direct determination of phospholipase D activity by infrared spectroscopy

Le Duy Do, René Buchet, Slawomir Pikula, Abdelkarim Abousalham, more

Analytical Biochemistry > 2012 > 430 > 1 > 32-38

To determine phospholipase D (PLD) activity, an infrared spectroscopy assay was developed, based on the phosphate vibrational mode of phospholipids such as dimyristoylphophatidylcholine (DMPC), lysophosphatidylglycerol (lysoPG), dipalmitoylphosphatidylethanolamine (DPPE), and lysophosphatidylserine (lysoPS). The phosphate bands served to monitor the hydrolysis rates of phospholipids with PLD. The...

article

Proteomic characterization of biogenesis and functions of matrix vesicles released from mineralizing human osteoblast-like cells

Cyril Thouverey, Agata Malinowska, Marcin Balcerzak, Agnieszka Strzelecka-Kiliszek, more

Journal of Proteomics > 2011 > 74 > 7 > 1123-1134

Matrix vesicles (MVs), released by budding from apical microvilli of osteoblasts during bone formation and development, are involved in the initiation of mineralization by promoting the formation of hydroxyapatite in their lumen. To gain additional insights into MV biogenesis and functions, MVs and apical microvilli were co-isolated from mineralizing osteoblast-like Saos-2 cells and their proteomes...

article

Synthesis and evaluation of thiophenyl derivatives as inhibitors of alkaline phosphatase

Lei Chang, Do Le Duy, Saida Mébarek, Florence Popowycz, more

Bioorganic & Medicinal Chemistry Letters > 2011 > 21 > 8 > 2297-2301

Pathological calcifications induced by deposition of basic phosphate crystals or hydroxyapatite (HA) on soft tissues are a large family of diseases comprising of ankylosing spondylitis (AS), end-stage osteoarthritis (OA) and vascular calcification. High activity of tissue non-specific alkaline phosphatase (TNAP) is a hallmark of pathological calcifications induced by HA deposition. The use of TNAP...

article

Acyl chain composition determines cardiolipin clustering induced by mitochondrial creatine kinase binding to monolayers

Ofelia Maniti, Mouhedine Cheniour, Marie-France Lecompte, Olivier Marcillat, more

BBA - Biomembranes > 2011 > 1808 > 4 > 1129-1139

It has been recently shown that mitochondrial creatine kinase (mtCK) organizes mitochondrial model membrane by modulating the state and fluidity of lipids and by promoting the formation of protein–cardiolipin clusters. This report shows, using Brewster angle microscopy, that such clustering is largely dependent on the acyl chain composition of phospholipids. Indeed, mtCK-cardiolipin domains were observed...

article

Characterization of caged compounds binding to proteins by NMR spectroscopy

Joanna Bandorowicz-Pikula, René Buchet, F. Javier Cañada, Martin Clémancey, more

Biochemical and Biophysical Research Communications > 2010 > 400 > 3 > 447-451

Photolysable caged ligands are used to investigate protein function and activity. Here, we investigate the binding properties of caged nucleotides and their photo released products to well established but evolutionary and structurally unrelated nucleotide-binding proteins, rabbit muscle creatine kinase (RMCK) and human annexin A6 (hAnxA6), using saturation transfer difference NMR spectroscopy. We...

article

Sinomenine, theophylline, cysteine, and levamisole: Comparisons of their kinetic effects on mineral formation induced by matrix vesicles

Lina Li, René Buchet, Yuqing Wu

Journal of Inorganic Biochemistry > 2010 > 104 > 4 > 446-454

The effects of sinomenine (SIN, an alkaloid extracted from the Chinese medicinal plant Sinomenium acutum used for centuries to treat rheumatic disease, including rheumatoid arthritis) on apatitic nucleation and matrix vesicle (MV)-induced mineral formation were compared with those of cysteine, levamisole, and theophylline. We found that SIN was not an inhibitor of tissue non-specific alkaline phosphatase...

article

Active creatine kinase is present in matrix vesicles isolated from femurs of chicken embryo: Implications for bone mineralization

Anna Sekrecka-Belniak, Marcin Balcerzak, Rene Buchet, Slawomir Pikula

Biochemical and Biophysical Research Communications > 2010 > 391 > 3 > 1432-1436

Proteomic analysis of matrix vesicles (MVs) isolated from 17-day-old chicken embryo femurs revealed the presence of creatine kinase. In this report we identified the enzyme functionally and suggest that the enzyme may participate in the synthesis of ATP from ADP and phosphocreatine within the lumen of these organelles. Then, ATP is converted by nucleotide hydrolyzing enzymes such as Na ⁺ ,...

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MINERALIZATION (11)
ALKALINE PHOSPHATASE (8)
MATRIX VESICLES (8)
INFRARED SPECTROSCOPY (4)
INHIBITOR (4)
HYDROXYAPATITE (3)
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BONE (2)
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OSTEOBLASTS (2)
PHOSPHATE (2)
PHOSPHOLIPASE D (2)
PHOSPHOLIPASE D (PLD) (2)
PYROPHOSPHATE (2)
TISSUE-NONSPECIFIC ALKALINE PHOSPHATASE (2)
VESICLES (2)
ANNEXIN VI; ATP[ET(PHNO 2 )]; FOURIER TRANSFORM INFRARED SPECTROSCOPY; PROTEIN SECONDARY STRUCTURE (1)
CA 2 + -ATPASE (1)
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ATP (1)
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CAGED LIGANDS (1)
CALCIUM-DEPENDENT BINDING (1)
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CD (1)
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Search results for: René Buchet

Three‐dimensional cell‐laden collagen scaffolds: From biochemistry to bone bioengineering

Effects of phospholipase D during cultured osteoblast mineralization and bone formation

Phospholipase D: A new mediator during high phosphate‐induced vascular calcification associated with chronic kidney disease

Lipid microenvironment affects the ability of proteoliposomes harboring TNAP to induce mineralization without nucleators

Collagen promotes matrix vesicle-mediated mineralization by vascular smooth muscle cells

Matrix vesicles from chondrocytes and osteoblasts: Their biogenesis, properties, functions and biomimetic models

Characteristics of minerals in vesicles produced by human osteoblasts hFOB 1.19 and osteosarcoma Saos-2 cells stimulated for mineralization

Functions of Rho family of small GTPases and Rho-associated coiled-coil kinases in bone cells during differentiation and mineralization

TNAP stimulates vascular smooth muscle cell trans-differentiation into chondrocytes through calcium deposition and BMP-2 activation: Possible implication in atherosclerotic plaque stability

Determination of phosphatase activity in osteoblasts by IR and two-dimensional correlation IR spectroscopy

Synthesis of benzofuran derivatives as selective inhibitors of tissue-nonspecific alkaline phosphatase: effects on cell toxicity and osteoblast-induced mineralization

Fatty acid composition in matrix vesicles and in microvilli from femurs of chicken embryos revealed selective recruitment of fatty acids

Inhibitors of tissue-nonspecific alkaline phosphatase: Design, synthesis, kinetics, biomineralization and cellular tests

Direct determination of phospholipase D activity by infrared spectroscopy

Proteomic characterization of biogenesis and functions of matrix vesicles released from mineralizing human osteoblast-like cells

Synthesis and evaluation of thiophenyl derivatives as inhibitors of alkaline phosphatase

Acyl chain composition determines cardiolipin clustering induced by mitochondrial creatine kinase binding to monolayers

Characterization of caged compounds binding to proteins by NMR spectroscopy

Sinomenine, theophylline, cysteine, and levamisole: Comparisons of their kinetic effects on mineral formation induced by matrix vesicles

Active creatine kinase is present in matrix vesicles isolated from femurs of chicken embryo: Implications for bone mineralization

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