Search results for: Brian J. Bahnson

Items from 1 to 20 out of 26 results

article

In vitro methanol production from methyl coenzyme M using the Methanosarcina barkeri MtaABC protein complex

Ming Dong, Tara D. Gonzalez, Meghan M. Klems, Lisa M. Steinberg, more

Biotechnology Progress > 33 > 5 > 1243 - 1249

Methanol:coenzyme M methyltransferase is an enzyme complex composed of three subunits, MtaA, MtaB, and MtaC, found in methanogenic archaea and is needed for their growth on methanol ultimately producing methane. MtaABC catalyzes the energetically favorable methyl transfer from methanol to coenzyme M to form methyl coenzyme M. Here we demonstrate that this important reaction for possible production...

article

A rationally designed mutant of plasma platelet-activating factor acetylhydrolase hydrolyzes the organophosphorus nerve agent soman

Stephen D. Kirby, Joseph Norris, Richard Sweeney, Brian J. Bahnson, more

Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics > 2015 > 1854 > 12 > 1809-1815

Organophosphorus compounds (OPs) such as sarin and soman are some of the most toxic chemicals synthesized by man. They exert toxic effects by inactivating acetylcholinesterase (AChE) and bind secondary target protein. Organophosphorus compounds are hemi-substrates for enzymes of the serine hydrolase superfamily. Enzymes can be engineered by amino acid substitution into OP-hydrolyzing variants (bioscavengers)...

article

Oligomeric state regulated trafficking of human platelet-activating factor acetylhydrolase type-II

Elizabeth S. Monillas, Jeffrey L. Caplan, Anastasia F. Thévenin, Brian J. Bahnson

BBA - Proteins and Proteomics > 2015 > 1854 > 5 > 469-475

The intracellular enzyme platelet-activating factor acetylhydrolase type-II (PAFAH-II) hydrolyzes platelet-activating factor and oxidatively fragmented phospholipids. PAFAH-II in its resting state is mainly cytoplasmic, and it responds to oxidative stress by becoming increasingly bound to endoplasmic reticulum and Golgi membranes. Numerous studies have indicated that this enzyme is essential for protecting...

article

Human paraoxonase double mutants hydrolyze V and G class organophosphorus nerve agents

Stephen D. Kirby, Joseph R. Norris, J. Richard Smith, Brian J. Bahnson, more

Chemico-Biological Interactions > 2013 > 203 > 1 > 181-185

Variants of human paraoxonase 1 (PON1) are being developed as catalytic bioscavengers for the organophosphorus chemical warfare agents (OP). It is preferable that the new PON1 variants have broad spectrum hydrolase activities to hydrolyze both G- and V-class OPs. H115W PON1 has shown improvements over wild type PON1 in its capacity to hydrolyze some OP compounds. We improved upon these activities...

article

Selective inhibitors and tailored activity probes for lipoprotein-associated phospholipase A2

Joseph M.G. Nagano, Ku-Lung Hsu, Landon R. Whitby, Micah J. Niphakis, more

Bioorganic & Medicinal Chemistry Letters > 2013 > 23 > 3 > 839-843

Lipoprotein-associated phospholipase A ₂ (Lp-PLA ₂ or PLA ₂ G7) binds to low-density lipoprotein (LDL) particles, where it is thought to hydrolyze oxidatively truncated phospholipids. Lp-PLA ₂ has also been implicated as a pro-tumorigenic enzyme in human prostate cancer. Several inhibitors of Lp-PLA ₂ have been described, including darapladib, which is currently...

article

77Se Enrichment of Proteins Expands the Biological NMR Toolbox

Stephanie A. Schaefer, Ming Dong, Renee P. Rubenstein, Wayne A. Wilkie, more

Journal of Molecular Biology > 2013 > 425 > 2 > 222-231

Sulfur, a key contributor to biological reactivity, is not amendable to investigations by biological NMR spectroscopy. To utilize selenium as a surrogate, we have developed a generally applicable ⁷⁷ Se isotopic enrichment method for heterologous proteins expressed in Escherichia coli. We demonstrate ⁷⁷ Se NMR spectroscopy of multiple selenocysteine and selenomethionine residues in...

article

Trafficking of Platelet-Activating Factor Acetylhydrolase Type II in Response to Oxidative Stress

Anastasia F. Thévenin, Elizabeth S. Monillas, Jason M. Winget, Kirk Czymmek, more

Biochemistry > 2011 > 50 > 39 > 8417 - 8426

article

Activation by phosphorylation and purification of human c-Jun N-terminal kinase (JNK) isoforms in milligram amounts

Anastasia F. Thévenin, Chati L. Zony, Brian J. Bahnson, Roberta F. Colman

Protein Expression and Purification > 2011 > 75 > 2 > 138-146

c-Jun N-terminal kinases (JNKs) are part of the mitogen-activated protein kinase (MAPK) signaling cascade. They are activated through dual phosphorylation of two residues in the activation loop, a threonine and a tyrosine, by MAP2 kinases (MKK4 and 7) in response to various extracellular stresses such as UV or osmotic shock, as well as by cytokines and growth factors. Only small amounts of phosphorylated,...

article

Structure of the Human Sulfhydryl Oxidase Augmenter of Liver Regeneration and Characterization of a Human Mutation Causing an Autosomal Recessive Myopathy,

Vidyadhar N. Daithankar, Stephanie A. Schaefer, Ming Dong, Brian J. Bahnson, more

Biochemistry > 2010 > 49 > 31 > 6737 - 6745

article

Structure of a premicellar complex of alkyl sulfates with the interfacial binding surfaces of four subunits of phospholipase A2

Ying H. Pan, Brian J. Bahnson

BBA - Proteins and Proteomics > 2010 > 1804 > 7 > 1443-1448

The properties of three discrete premicellar complexes (E ₁ ^# , E ₂ ^# , E ₃ ^# ) of pig pancreatic group-IB secreted phospholipase A2 (sPLA ₂ ) with monodisperse alkyl sulfates have been characterized [Berg, O. G. et al., Biochemistry 43, 7999–8013, 2004]. Here we have solved the 2.7Å crystal structure of group-IB sPLA ₂ complexed with 12 molecules...

article

Crystal Structure of Human Senescence Marker Protein 30: Insights Linking Structural, Enzymatic, and Physiological Functions,

Subhendu Chakraborti, Brian J. Bahnson

Biochemistry > 2010 > 49 > 16 > 3436 - 3444

article

Crystal structures of human group-VIIA phospholipase A2 inhibited by organophosphorus nerve agents exhibit non-aged complexes

Uttamkumar Samanta, Stephen D. Kirby, Prabhavathi Srinivasan, Douglas M. Cerasoli, more

Biochemical Pharmacology > 2009 > 78 > 4 > 420-429

The enzyme group-VIIA phospholipase A2 (gVIIA-PLA2) is bound to lipoproteins in human blood and hydrolyzes the ester bond at the sn-2 position of phospholipid substrates with a short sn-2 chain. The enzyme belongs to a serine hydrolase superfamily of enzymes, which react with organophosphorus (OP) nerve agents. OPs ultimately exert their toxicity by inhibiting human acetycholinesterase at nerve synapses,...

article

Crystal Structures of Brain Group-VIII Phospholipase A2 in Nonaged Complexes with the Organophosphorus Nerve Agents Soman and Sarin ^† ^, ^‡

Todd M. Epstein, Uttamkumar Samanta, Stephen D. Kirby, Douglas M. Cerasoli, more

Biochemistry > 2009 > 48 > 15 > 3425 - 3435

article

Structural Basis for Bile Salt Inhibition of Pancreatic Phospholipase A2

Ying H. Pan, Brian J. Bahnson

Journal of Molecular Biology > 2007 > 369 > 2 > 439-450

Bile salt interactions with phospholipid monolayers of fat emulsions are known to regulate the actions of gastrointestinal lipolytic enzymes in order to control the uptake of dietary fat. Specifically, on the lipid/aqueous interface of fat emulsions, the anionic portions of amphipathic bile salts have been thought to interact with and activate the enzyme group-IB phospholipase A2 (PLA2) derived from...

article

The interfacial binding surface of phospholipase A2s

Jason M. Winget, Ying H. Pan, Brian J. Bahnson

BBA - Molecular and Cell Biology of Lipids > 2006 > 1761 > 11 > 1260-1269

For membrane-associated enzymes, which access substrate from either a monolayer or bilayer of the aggregate substrate, the partitioning from the aqueous phase to this phospholipid interface is critical for catalysis. Despite a large and expanding body of knowledge regarding interfacial enzymes, the biophysical steps involved in interfacial recognition and adsorption remain relatively poorly understood...

article

Roles of Additives and Precipitants in Crystallization of Calcium- and Integrin-Binding Protein

Bryan W. Berger, Chad J. Blamey, Ulhas P. Naik, Brian J. Bahnson, more

Crystal Growth & Design > 2005 > 5 > 4 > 1499 - 1507

article

Kinetic and Structural Properties of Disulfide Engineered Phospholipase A ₂ : Insight into the Role of Disulfide Bonding Patterns ^† ^, ^‡

Bao-Zhu Yu, Ying H. Pan, Marcel J. W. Janssen, Brian J. Bahnson, more

Biochemistry > 2005 > 44 > 9 > 3369 - 3379

article

Structure, function and interfacial allosterism in phospholipase A2: insight from the anion-assisted dimer

Brian J. Bahnson

Archives of Biochemistry and Biophysics > 2005 > 433 > 1 > 96-106

Enzymes that function on membrane surfaces offer many challenges to understanding structural and functional details due to the difficulties of obtaining relevant information of the protein in a physiological environment. Focusing on this aspect of structural biology, it is important to develop conditions that mimic the interaction of membrane proteins with their binding surface and ultimately the...

article

Structure/function analyses of human serum paraoxonase (HuPON1) mutants designed from a DFPase-like homology model

David T. Yeung, Denis Josse, James D. Nicholson, Akhil Khanal, more

BBA - Proteins and Proteomics > 2004 > 1702 > 1 > 67-77

Human serum paraoxonase (HuPON1) is a calcium-dependent enzyme that hydrolyzes esters, including organophosphates and lactones, and exhibits anti-atherogenic properties. A few amino acids have been shown to be essential for the enzyme's arylesterase and organophosphatase activities. Until very recently, a three-dimensional model was not available for HuPON1, so functional roles have not been assigned...

article

Crystal Structure and Amide H/D Exchange of Binary Complexes of Alcohol Dehydrogenase from Bacillus stearothermophilus : Insight into Thermostability and Cofactor Binding ^† ^, ^‡

Christopher Ceccarelli, Zhao-Xun Liang, Michael Strickler, Gerd Prehna, more

Biochemistry > 2004 > 43 > 18 > 5266 - 5277

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Keywords

INTERFACIAL ENZYME (4)
PHOSPHOLIPASE A2 (3)
MEMBRANE ASSOCIATED PROTEIN (2)
NERVE AGENT (2)
PARAOXONASE (2)
77SE NMR (1)
ALLOSTERIC (1)
ANAEROBIC METHANE OXIDATION (1)
ARYLESTERASE (1)
AUGMENTER OF LIVER REGENERATION (1)
BILE SALT (1)
BIOSCAVENGER (1)
C-JUN N-TERMINAL KINASES (1)
CALCIUM (1)
CALCIUM ESTERASE (1)
COM (1)
CORRINOID (1)
CRYSTALLOGRAPHY (1)
ESTERASE (1)
HOMOLOGY MODEL (1)
HUMAN (1)
HUPON1 (1)
I-FACE (1)
INHIBITION (1)
INHIBITOR (1)
LIPASE (1)
LIPID HOMEOSTASIS (1)
LP-PLA2 (1)
MAP KINASE (1)
MECHANISM (1)
MEMBRANE PROTEIN (1)
METHANOGENS (1)
METHANOL FROM METHANE (1)
MYRISTOYLATION (1)
NERVE AGENTS (1)
OLIGOMERIC STATE (1)
OP (1)
ORGANOPHOSPHATASE (1)
ORGANOPHOSPHATE (1)
ORGANOPHOSPHORUS (1)
ORGANOPHOSPHORUS COMPOUNDS (1)
OXIDATIVE STRESS (1)
PAF-AH (1)
PARAOXON (1)
PERIPHERAL MEMBRANE PROTEIN (1)
PHOSPHOLIPASE (1)
PHOSPHORYLATION (1)
PLA2 (1)
PLASMA PLATELET-ACTIVATING FACTOR ACETYLHYDROLASE (1)
PON1 (1)
PREMICELLAR COMPLEX (1)
PROCESSIVE TURNOVER (1)
PROTEOMICS (1)
SCREENING (1)
SELENIUM NMR (1)
SELENOCYSTEINE (1)
SELENOPROTEINS (1)
SIGNALING CASCADE (1)
SOMAN (1)
SPLA2, ALKYL SULFATE (1)
TRAFFICKING (1)
ZN ENZYME (1)
Β-PROPELLER (1)
more

Data set

Elsevier (13)
ACS (12)
Wiley (1)

Journal

Biochemistry (11)
BBA - Proteins and Proteomics (3)
Journal of Molecular Biology (2)
Archives of Biochemistry and Biophysics (1)
BBA - Molecular and Cell Biology of Lipids (1)
Biochemical Pharmacology (1)
Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics (1)
Bioorganic & Medicinal Chemistry (1)
Bioorganic & Medicinal Chemistry Letters (1)
Biotechnology Progress (1)
Chemico-Biological Interactions (1)
Crystal Growth & Design (1)
Protein Expression and Purification (1)
more

INFONA - science communication portal

Search results for: Brian J. Bahnson

In vitro methanol production from methyl coenzyme M using the Methanosarcina barkeri MtaABC protein complex

A rationally designed mutant of plasma platelet-activating factor acetylhydrolase hydrolyzes the organophosphorus nerve agent soman

Oligomeric state regulated trafficking of human platelet-activating factor acetylhydrolase type-II

Human paraoxonase double mutants hydrolyze V and G class organophosphorus nerve agents

Selective inhibitors and tailored activity probes for lipoprotein-associated phospholipase A2

77Se Enrichment of Proteins Expands the Biological NMR Toolbox

Trafficking of Platelet-Activating Factor Acetylhydrolase Type II in Response to Oxidative Stress

Activation by phosphorylation and purification of human c-Jun N-terminal kinase (JNK) isoforms in milligram amounts

Structure of the Human Sulfhydryl Oxidase Augmenter of Liver Regeneration and Characterization of a Human Mutation Causing an Autosomal Recessive Myopathy,

Structure of a premicellar complex of alkyl sulfates with the interfacial binding surfaces of four subunits of phospholipase A2

Crystal Structure of Human Senescence Marker Protein 30: Insights Linking Structural, Enzymatic, and Physiological Functions,

Crystal structures of human group-VIIA phospholipase A2 inhibited by organophosphorus nerve agents exhibit non-aged complexes

Crystal Structures of Brain Group-VIII Phospholipase A2 in Nonaged Complexes with the Organophosphorus Nerve Agents Soman and Sarin ^† ^, ^‡

Structural Basis for Bile Salt Inhibition of Pancreatic Phospholipase A2

The interfacial binding surface of phospholipase A2s

Roles of Additives and Precipitants in Crystallization of Calcium- and Integrin-Binding Protein

Kinetic and Structural Properties of Disulfide Engineered Phospholipase A ₂ : Insight into the Role of Disulfide Bonding Patterns ^† ^, ^‡

Structure, function and interfacial allosterism in phospholipase A2: insight from the anion-assisted dimer

Structure/function analyses of human serum paraoxonase (HuPON1) mutants designed from a DFPase-like homology model

Crystal Structure and Amide H/D Exchange of Binary Complexes of Alcohol Dehydrogenase from Bacillus stearothermophilus : Insight into Thermostability and Cofactor Binding ^† ^, ^‡

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