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BACKGROUNDThe authors developed an ex vivo methodology to perform drug library screening against human leukemia.
METHODSThe strategy for this screening relied on human blood or bone marrow cultures under hypoxia; under these conditions, leukemia cells deplete oxygen faster than normal cells, causing a hemoglobin oxygenation shift. Several advantages were observed: 1) partial recapitulation of the...
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