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Genotyping of single nucleotide polymorphisms (SNPs) allows diagnosis of human genetic disorders associated with single base mutations. Conventional SNP genotyping methods are capable of providing either accurate or high-throughput detection, but are still labor-, time-, and resource-intensive. Microfluidics has been applied to SNP detection to provide fast, low-cost, and automated alternatives, although...
This paper presents an integrated, MEMS-based approach to the detection of single nucleotide polymorphisms (SNPs). This SNP detection approach aims to eliminate complicated fluidic control operations by using a single-chamber microfluidic device, which incorporates polymerase chain reaction (PCR) and single base extension (SBE) on microbeads. Experimental results demonstrate that the device is capable...
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