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Stimulated Raman scattering (SRS) microscopy [1] appears nowadays as a viable platform with strong potential in biology and medicine. The implementation of SRS is generally accompanied by parasitic signals stemming from other quasi-instantaneous nonlinear optical processes such as two-photon absorption, cross phase modulation (XPM), and thermal effects. Implementations which involve propagation of...
Coherent Raman imaging by means of coherent anti-Stokes Raman scattering (CARS) or stimulated Raman scattering (SRS) have been promoted from almost two decades now as efficient label free fast imaging modalities with chemical sensitivity and with a sensitivity orders of magnitude greater than spontaneous Raman scattering [1]. The ability of CARS to detect stable isotope (e.g. deuterium and C13) is...
We demonstrate the use of an acousto-optic programmable dispersive filter (AOPDF) as a fast delay line for pump-probe spectroscopy and stimulated Raman scattering. Both of these nonlinear imaging schemes have exhibited potential for various applications including cancer detection.
Obtaining information on the matter organization on the micrometer scale still remains a challenge in physics, chemistry or biology. Non-linear vibrational processes such as Coherent Anti-Stokes Raman Scattering (CARS) are powerful tools for 3D imaging of chemical properties without sample preparation. The CARS process is a stimulated Raman process based on the interaction of 2 photons, called pump...
To get insights into the molecular interactions that take place in cells and tissues, fluorescence microscopy appears nowadays as the most powerful and common way to proceed. Advances in fluorescent fusion proteins such as green fluorescent protein (GFP), chemical dyes and nanoparticles provide the biologist with a rich labeling kit that is at the very basis of fluorescence microscopy. Although extremely...
We report a step toward scanning endomicroscopy without distal optics. Indeed, we realized a video-rate lensless point scanning endoscope where the size of the endoscope distal probe was reduced to the fundamental limit — the 400 μm diameter of the endoscope fiber itself. Following in the footsteps of coherent laser beam combining, the focusing of the beam at the distal end of a fiber bundle is achieved...
Plasmonic nanoantennas are promising tools to control and manipulate optical fields at the nanometre scale [1,2]. In this work, we introduce a novel type of plasmonic nanoantenna especially designed for enhanced single molecule analysis in solutions at high concentrations. The nanoantenna is based on a dimer made of two gold half-spheres surrounded by a box aperture and is milled in a gold film by...
We experimentally observe and measure the spectral phase shift of a pulse subjected to spectral focusing. We find a phase shift of π/2, reaffirming the Gouy phase shift as a general consequence of wave confinement whether in space/momentum or frequency/time coordinates.
We measure the quadratic nonlinear optical properties, namely second harmonic generation (SHG) and two-photon excitation fluorescence (TPEF), of nanocrystals of different derivatives of pyrazoline. The derivatives of pirazoline, like for example DCNP are supposed to have high second order nonlinear susceptibility [1, 2]. We use a nonlinear optical microscopy setup, samples were excited with a femtosecond...
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