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A novel immunoassay channeling sensor for α-fetoprotein (AFP) was proposed by incorporating biorecognition molecules into the mesopores of well-ordered hexagonal SBA-15. As the biosensing element, alkaline phosphatase (ALP)-labeled antibody was embedded into the mesopores with the substrate of 1-naphthyl phosphate (1-NP). The encapsulated ALP-labeled antibody retained its bioactivities on catalytic...
A novel strategy for the fabrication of sensitive immunosensor to detect α-fetoprotein (AFP) in human serum has been proposed. The immunosensor was prepared by immobilizing AFP antigen onto the glassy carbon electrode (GC) modified by gold nanoparticles and carbon nanotubes doped chitosan (GNP/CNT/Ch) film. GNP/CNT hybrids were produced by one-step synthesis based on the direct redox reaction. The...
By introducing heterocyclic compound to immunoassay system as an electrochemical substrate for the fist time, a new voltammetric enzyme-linked immunoassay system of 3-hydroxyl-2-aminopyridine (HAP)-H 2 O 2 -horseradish peroxidase (HRP) has been developed. HAP was oxidized with H 2 O 2 catalyzed by HRP, and the resulting electroactive product produced a sensitive voltammetric...
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