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The reactivity of mitochondrial DNA (mtDNA) sequences from Torulopsis glabrata and Saccharomyces cerevisiae towards single-strand-specific nucleases has been examined. AT-rich stretches located in intergenic sequences from both yeasts were cleaved by nucleases when the sequences were contained in supercoiled plasmid DNA. In particular ori/rep sequences from the mtDNA of S. cerevisiae were shown to...
A mutant mitochondrial DNA of Saccharomyces cerevisiae strain R 0.54 has been shown to have a 5 kb deletion which removes the oril sequence and its surrounding intergenic sequences between the pro tRNA and 15 S rRNA genes. The deleted DNA has been sequenced and is found to be flanked by two 11 bp direct repeats. The deletion mutant displays a normal phenotype in non-fermentable media. This implies...
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