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Purpose
To rapidly identify protein abundance changes in biopsy‐level fresh‐frozen hepatocellular carcinoma (HCC).
Experimental design
The pressure‐cycling technology (PCT) is applied and sequential window acquisition of all theoretical mass spectra (SWATH‐MS) workflow is optimized to analyze 38 biopsy‐level tissue samples from 19 HCC patients. Each proteome is analyzed with 45 min LC gradient...
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