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A cellular membrane affinity chromatography (CMAC) column has been created using cellular membrane fragments from a 1321N1 cell line stably transfected with the P2Y 1 receptor. The CMAC(1321N1 P2Y1 ) column contained functional P2Y 1 and histamine 1 receptors, which independently bound receptor-specific ligands. The data obtained with the CMAC(1321N1 P2Y1 ) column demonstrate...
CMAC (cellular membrane affinity chromatography columns) have been developed for the study of the human multidrug transporters MRP1, MRP2 and the breast cancer resistance protein (BCRP). The columns were constructed using the immobilized artificial membrane (IAM) stationary phase and cellular membrane fragments obtained from Spodoptera frugiperda (Sf9) cells that had been stably transfected with human...
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