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An antifungal protein was isolated from the intercellular washing fluid (IWF) of leaves of sugar beet (Beta vulgaris L., cv. Monova) and purified to homogeneity. The protein, IWF6, comprising 37 amino acids with six cysteines, was able to inhibit the growth of the pathogen Cercospora beticola (Sacc.) in vitro, by 75% after 120 h of growth at a concentration of 20 μg ml -1 . The amino...
A novel protein (IWF5) comprising 92 amino acids has been purified from the intercellular washing fluid of sugar beet leaves using cation exchange chromatography and reversed phase high performance liquid chromatography. Based on amino acid sequence homology, including the presence of eight cysteines at conserved positions, the protein can be classified as a member of the plant family of non-specific...
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