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The potential for insect resistance to the spinosyns, a novel class of insecticide chemistry, was examined using a laboratory strain of the tobacco budworm, Heliothis virescens (F.), originally collected from tobacco at sites in North Carolina. Technical grade spinosad (spinosyns A and D), was topically applied to third instars. Initially 533 third instars were used but one to two thousand larvae...
Bioassay methodology was developed for species diagnosis of Heliothis virescens compared with Helicoverpa zea in cotton and to detect H. virescens larvae with significant levels of resistance to the biopesticide, Bacillus thuringiensis. The assay end-point is feeding disruption, which is measured by a lack of fecal production by larvae exposed to a diagnostic dose of CryIAc in a blue indicator diet...
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