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Response surface methodology was employed to enhance the anaerobic production of rhamnolipid by recombinant Pseudomonas stutzeri Rhl. Glycerol is a promising carbon source used to anaerobically produce rhamnolipid. In a Plackett–Burman design, glycerol, KH2PO4 and yeast extract were significant factors. The proposed optimized medium contained the following: 46·55 g l−1 glycerol; 3 g l−1 NaNO3; 5·25 g l...
Recombinant glucose oxidase from Aspergillus niger expressed in Pichia pastoris by fed‐batch fermentation was purified and assessed with 1·26 purification fold to homogeneity using Q‐Sepharose F.F. chromatography. The enzyme was determined by SDS‐PAGE and gradient PAGE, which showed a dimeric form of 150 kDa. The purified rGOD was proved to be a glycoprotein, and the content of which was estimated...
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