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Energy‐dependent translational throttle A (EttA) from Escherichia coli is a paradigmatic ABC‐F protein that controls the first step in polypeptide elongation on the ribosome according to the cellular energy status. Biochemical and structural studies have established that ABC‐F proteins generally function as translation factors that modulate the conformation of the peptidyl transferase center upon...
Despite the great importance of human membrane proteins involved in detoxification mechanisms, their wide use for biochemical approaches is still hampered by several technical difficulties considering eukaryotic protein expression in order to obtain the large amounts of protein required for functional and/or structural studies. Lactococcus lactis has emerged recently as an alternative heterologous...
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