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DNA is the crucial material foundation for survival, reproduction and evolutionary development of species. DNA sequences data compression plays an important role in storage, management and transmission of massive genetic data. In the field of data compression, there are not only classical data compression algorithms but also some improved algorithms that are specific to DNA sequences data compression...
The cDNA of DREB transcription factor named OjDREB1 was obtained from Lilyturf (Ophiopogon japonicus) under the drought stress of 12h by RT-PCR method. Sequence analysis showed that cDNA of OjDREB1 is 1779 bp long, the open reading frame is 1062bp.The predicted protein has 353 amino acids with an estimated molecular mass of 38.69 kDa and an isoelectric point of 6.46. The deduced primary structure...
This study was aimed to isolate ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit (rbcL) from lilyturf (Ophiopogon japonicus). A pair of primers used in amplifying chloroplast fragment from lilyturf was designed according to the corresponding sequences in tobacco, spinach chloroplast genomes. The fragment containing rbcL gene was cloned from lilyturf chloroplast genome. Sequencing analysis...
This study aimed to study the immune activity of glutamate dehydrogenase (GDH) of streptococcus suis serotype 2 and detect its activity. METHODS: The GDH gene was cloned and sequenced. Recombinant GDH protein was expressed in a prokaryotic expression system and purified by affinity chromatography. Immunocompetenc of the resulting protein was analyzed by western blotting with anti-GDH monoclonal antibody...
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