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Virgin queens of A. florea were produced in 10 queenless colonies yielding 106 queens with an average of 10.6 ± 2.99 queen cells per colony and a success rate of 65.23 ± 0.14% virgin queens. Spermatozoa were collected directly from the seminal vesicles. Thirty queens were inseminated, each with a pool of about 3.12 × 106 spermatozoa derived from 8 drones. Six queens began to lay eggs 5 to 14 days...
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