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To improve the vitrification protocol for Hypericum perforatum shoot tips, calorimetric measurements of the intensity of thermal transitions in the samples during warming using differential scanning calorimetry were assessed. Comparison of ABA treatment and cold-acclimation revealed higher content of crystallizable water in the latter. Regeneration capacity of cryopreserved H. perforatum shoot tips...
It has been well documented that unlike in vitrification during slow cooling at least two major factors, dehydration and ice crystal formation and their growth affect the survival rate of cryopreserved plant material. Presented results with shoot tips of medicinal plant species Hypericum perforatum show negative correlation between cooling rate and recovery after cryopreservation with a maximum of...
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