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This paper presents a digital repetitive control design on the basis of attracting law (AL). A control law, together with the disturbance compensation, can be derived by applying the AL which includes a measure of disturbance-rejection. Chattering is alleviated by a sign-function continuous approximation technique. To characterize the closed-loop performance, the steady-state error band, absolute...
This paper deals with the problem of repetitive control for discrete-time systems on the basis of ideal error dynamics together with periodic disturbance compensation. By replacing the sign function in an attracting law with the saturation function, a repetitive controller is designed, where the chattering problem is avoided and the periodic disturbances can be rejected completely. The steady-state...
This paper deals with the problem of repetitive control for single-input single-output discrete-time systems. An ideal error dynamics is formed by using a discrete attracting law, and the repetitive control law is derived based on the ideal error dynamics. The compensation for periodic disturbances is involved, and tracking performance could be improved so that the periodic disturbances are rejected...
We present a protocol with isothermal rolling-circle amplification (RCA) to detect DNA point mutation on chip. The basic principle of the method is an allele-specific oligonucleotide circularization mediated by special DNA ligase. The probe is circularized when perfect complementary sequences between the probe oligonucleotide and target DNA. Mismatches around the ligation site can prevent probe circularization...
The amylomaltase gene of the Streptomyces ST66 was cloned. The open reading frame of this gene consisted of 2,136 nucleotides and encoded a polypeptide that was711 amino acids. The amylomaltase gene was overexpressed in E. coli. The detailed action profile of crude enzyme from E.coli Top 10F'/pTrc-CKS- MalQ on amylose was analyzed and tested whether this crude enzyme could catalyze cyclization of...
Listeriamonocytogenes (LM) is a bacterial foodborne pathogen responsible for listeriosis, an illness characterized by encephalitis, septicaemia , and meningitis. One of the best ways to detect and confrim the pathogen is through the detection of one of the virulence factors, listeriolysin O (LLO) produced by the microorganism. LLO is absolutely required for virulence by L. monocytogenes, and is found...
A novel assay for the cyclic voltammetry (CV) detection of PCR amplification to using electrochemical DNA biosensor was reported. The coupling of DNA electrochemical sensors have specialities of quick detection and cheapness and have the potential of the quantitative evaluation of the PCR amplification. The electrochemical sensor was obtained by immobilizing single-stranded oligonucleotides onto gold...
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