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Citrus tristeza virus (CTV) occurs in most citrus producing regions of the world, and it is the most serious viral pathogen of citrus. With the recent establishment of the brown citrus aphid, Toxoptera citricida, its most efficient vector, on Madeira Island (Portugal) and in Florida (USA) and the countries of the Caribbean Basin, the impact of CTV is likely to increase in these regions. Since there...
introduction. The Elisa technique has been the method of choice for diagnosis of citrus tristeza virus (CTV) during recent years. However, it was sometimes reported to be not sensitive enough to detect the virus before the onset of decline symptoms. The development of diagnostic methods based on genome amplification appears very attractive: recent methods of DNA amplification in vitro, such as...
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