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Rabbit hemorrhagic disease virus (RHDV) is an important member of the caliciviridae family. Currently, no suitable tissue culture system is available for proliferating RHDV, which limits the study of its pathogenesis. To bypass this obstacle, we established a cell line, RK13-VPg, stably expressing the VPg gene with a lentivirus packaging system in this study. In addition, the recently constructed...
In this study, virus-like particles (VLPs) derived from rabbit hemorrhagic disease virus (RHDV) were evaluated for the development of a vaccine against RHDV infection. The VP60 gene was cloned and inserted into a pSMK expression vector containing a small ubiquitin-like modifier (SUMO) tag that can promote the soluble expression of heterologous proteins in Escherichia coli cells. After expression and...
Non-standardization of messages when using IEC61968 messages to fulfill information integration of DMS often causes interoperation failures and affects related works. Effective rules and methods to test IEC61968 messages are deficient now. To solve this problem, layered rules along with a metadata & rule-driven framework of IEC61968 message conformance test are proposed. An extensible & loosely-coupled...
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