Asish K. Saha

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Lipopolysaccharide (LPS)-induced septic shock causes profound changes in myocardial energy metabolites in pigs

Joaquin Lado-Abeal, Noelia Martinez-Sánchez, Jose Angel Cocho, Manuel Martín-Pastor, more

Metabolomics > 2018 > 14 > 10 > 1-12

Introduction Energy deficiency is a cause for myocardial dysfunction during septic shock. In rodents, septic shock decreases the oxidation of long-chain fatty acids and glucose in the myocardium causing energy deficiency. However, the effect of septic shock on myocardial energy metabolites in large animals and human is unknown. Objectives Investigate the effects of septic shock on myocardial energy...

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The effects of troglitazone on AMPK in HepG2 cells

Katherine M. Allen, Kimberly A. Coughlan, Fabliha N. Mahmood, Rudy J. Valentine, more

Archives of Biochemistry and Biophysics > 2017 > 623-624 > C > 49-57

AMP-activated protein kinase (AMPK) is an enzyme crucial in cellular metabolism found to be inhibited in many metabolic diseases including type 2 diabetes. Thiazolidinediones (TZDs) are a class of anti-diabetic drug known to activate AMPK through increased phosphorylation at Thr¹⁷², however there has been no research to date on whether they have any effect on inhibition of AMPK's lesser known site...

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Insulin inhibits AMPK activity and phosphorylates AMPK Ser485/491 through Akt in hepatocytes, myotubes and incubated rat skeletal muscle

Rudy J. Valentine, Kimberly A. Coughlan, Neil B. Ruderman, Asish K. Saha

Archives of Biochemistry and Biophysics > 2014 > 562 > Complete > 62-69

Recent studies have highlighted the importance of an inhibitory phosphorylation site, Ser^485/491, on the α-subunit of AMP-activated protein kinase (AMPK); however, little is known about the regulation of this site in liver and skeletal muscle. We examined whether the inhibitory effects of insulin on AMPK activity may be mediated through the phosphorylation of this inhibitory Ser^485/491 site in hepatocytes,...

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Estradiol Regulates Brown Adipose Tissue Thermogenesis via Hypothalamic AMPK

Pablo B. Martínez de Morentin, Ismael González-García, Luís Martins, Ricardo Lage, more

Cell Metabolism > 2014 > 20 > 1 > 41-53

Estrogens play a major role in the modulation of energy balance through central and peripheral actions. Here, we demonstrate that central action of estradiol (E2) inhibits AMP-activated protein kinase (AMPK) through estrogen receptor alpha (ERα) selectively in the ventromedial nucleus of the hypothalamus (VMH), leading to activation of thermogenesis in brown adipose tissue (BAT) through the sympathetic...

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SIRT4 Coordinates the Balance between Lipid Synthesis and Catabolism by Repressing Malonyl CoA Decarboxylase

Gaëlle Laurent, Natalie J. German, Asish K. Saha, Vincent C.J. de Boer, more

Molecular Cell > 2013 > 50 > 5 > 686-698

Lipid metabolism is tightly controlled by the nutritional state of the organism. Nutrient-rich conditions increase lipogenesis, whereas nutrient deprivation promotes fat oxidation. In this study, we identify the mitochondrial sirtuin, SIRT4, as a regulator of lipid homeostasis. SIRT4 is active in nutrient-replete conditions to repress fatty acid oxidation while promoting lipid anabolism. SIRT4 deacetylates...

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The evolution of insulin resistance in muscle of the glucose infused rat

Amanda E. Brandon, Andrew J. Hoy, Lauren E. Wright, Nigel Turner, more

Archives of Biochemistry and Biophysics > 2011 > 509 > 2 > 133-141

Glucose infusion into rats causes skeletal muscle insulin resistance that initially occurs without changes in insulin signaling. The aim of the current study was to prolong glucose infusion and evaluate other events associated with the transition to muscle insulin resistance. Hyperglycemia was produced in rats by glucose infusion for 3, 5 and 8h. The rate of infusion required to maintain hyperglycemia...

article

Ablation of ARNT/HIF1β in Liver Alters Gluconeogenesis, Lipogenic Gene Expression, and Serum Ketones

Xiaohui L. Wang, Ryo Suzuki, Kevin Lee, Thien Tran, more

Cell Metabolism > 2009 > 9 > 6 > 565

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Ablation of ARNT/HIF1β in Liver Alters Gluconeogenesis, Lipogenic Gene Expression, and Serum Ketones

Xiaohui L. Wang, Ryo Suzuki, Kevin Lee, Thien Tran, more

Cell Metabolism > 2009 > 9 > 5 > 428-439

We have previously shown that expression of the transcription factor ARNT/HIF1β is reduced in islets of humans with type 2 diabetes. We have now found that ARNT is also reduced in livers of diabetics. To study the functional effect of its reduction, we created mice with liver-specific ablation (L-ARNT KO) using ARNT loxP mice and adenoviral-mediated delivery of Cre. L-ARNT KO mice had normal blood...

article

Concurrent regulation of AMP-activated protein kinase and SIRT1 in mammalian cells

Gabriela Suchankova, Lauren E. Nelson, Zachary Gerhart-Hines, Meghan Kelly, more

Biochemical and Biophysical Research Communications > 2009 > 378 > 4 > 836-841

We examined in HepG2 cells whether glucose-induced changes in AMP-activated protein kinase (AMPK) activity could be mediated by SIRT1, an NAD ⁺ -dependent histone/protein deacetylase that has been linked to the increase in longevity caused by caloric restriction. Incubation with 25 vs. 5mM glucose for 6h concurrently diminished the phosphorylation of AMPK (Thr 172) and ACC (Ser 79), increased...

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The AMPK-Malonyl-CoA-CPT1 Axis in the Control of Hypothalamic Neuronal Function—Reply

Miguel López, Asish K. Saha, Carlos Diéguez, Antonio Vidal-Puig

Cell Metabolism > 2008 > 8 > 3 > 176

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Hypothalamic Fatty Acid Metabolism Mediates the Orexigenic Action of Ghrelin

Miguel López, Ricardo Lage, Asish K. Saha, Diego Pérez-Tilve, more

Cell Metabolism > 2008 > 7 > 5 > 389-399

Current evidence suggests that hypothalamic fatty acid metabolism may play a role in regulating food intake; however, confirmation that it is a physiologically relevant regulatory system of feeding is still incomplete. Here, we use pharmacological and genetic approaches to demonstrate that the physiological orexigenic response to ghrelin involves specific inhibition of fatty acid biosynthesis induced...

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AMPK regulation of the growth of cultured human keratinocytes

Asish K. Saha, Kelly Persons, Joshua D. Safer, Zhijun Luo, more

Biochemical and Biophysical Research Communications > 2006 > 349 > 2 > 519-524

AMP kinase (AMPK) is a fuel sensing enzyme that responds to cellular energy depletion by increasing processes that generate ATP and inhibiting others that require ATP but are not acutely necessary for survival. In the present study, we examined the relationship between AMPK activation and the growth (proliferation) of cultured human keratinocytes and assessed whether the inhibition of keratinocyte...

article

Malonyl-CoA decarboxylase is present in the cytosolic, mitochondrial and peroxisomal compartments of rat hepatocytes

Erik Joly, Moise Bendayan, Raphaël Roduit, Asish K. Saha, more

FEBS Letters > 2005 > 579 > 29 > 6581-6586

A role for cytosolic malonyl-CoA decarboxylase (MCD) as a regulator of fatty acid oxidation has been postulated. However, there is no direct evidence that MCD is present in the cytosol. To address this issue, we performed cell fractionation and electron microscopic colloidal gold studies of rat liver to determine the location and activity of MCD. By both methods, substantial amounts of MCD protein...

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AMPK, the metabolic syndrome and cancer

Zhijun Luo, Asish K. Saha, Xiaoqin Xiang, Neil B. Ruderman

Trends in Pharmacological Sciences > 2005 > 26 > 2 > 69-76

The fuel-sensing enzyme 5′-AMP-activated protein kinase (AMPK) has a major role in the regulation of cellular lipid and protein metabolism in response to stimuli such as exercise, changes in fuel availability and the adipocyte-derived hormones leptin and adiponectin. Recent studies indicate that abnormalities in cellular lipid metabolism are involved in the pathogenesis of the metabolic syndrome,...

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Dietary polyunsaturated fatty acids enhance hepatic AMP-activated protein kinase activity in rats

Gabriela Suchankova, Michael Tekle, Asish K. Saha, Neil B. Ruderman, more

Biochemical and Biophysical Research Communications > 2005 > 326 > 4 > 851-858

Polyunsaturated fatty acids (PUFA) and a number of drugs (metformin, thiazolidinediones) and hormones (leptin, adiponectin) that activate AMP-activated protein kinase (AMPK) have been reported to improve insulin sensitivity. To determine whether PUFA activate AMPK, Sprague–Dawley rats were adapted to a 3h meal-feeding regimen using a fat-free diet (FFD) supplemented with fish oil (n−3) or triolein...

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AMP-activated protein kinase activators can inhibit the growth of prostate cancer cells by multiple mechanisms

Xiaoqin Xiang, Asish K. Saha, Rong Wen, Neil B. Ruderman, more

Biochemical and Biophysical Research Communications > 2004 > 321 > 1 > 161-167

Prostate cancer cells require high rates of de novo fatty acid synthesis and protein synthesis for their rapid growth. We report here that the growth of these cells is markedly diminished by incubation with activators of AMP-activated protein kinase (AMPK), a fuel-sensing enzyme that has been shown to diminish both of these processes in intact tissues. Inhibition of cell growth was observed when AMPK...

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AMPK activity is diminished in tissues of IL-6 knockout mice: the effect of exercise

Meghan Kelly, Charlotte Keller, Paco R. Avilucea, Pernille Keller, more

Biochemical and Biophysical Research Communications > 2004 > 320 > 2 > 449-454

Following exercise, AMP-activated protein kinase (AMPK) activity is increased several fold in rat liver and adipose tissue as well as muscle; however, the mechanism by which this occurs is not known. Interleukin-6 (IL-6) is released from muscle in large amounts during and after sustained physical activity resulting in up to 100-fold increases in its plasma concentration, from 1-2ng/ml to 50-100ng/ml...

article

Pioglitazone treatment activates AMP-activated protein kinase in rat liver and adipose tissue in vivo

Asish K. Saha, Paco R. Avilucea, Ji-Ming Ye, Murwarid M. Assifi, more

Biochemical and Biophysical Research Communications > 2004 > 314 > 2 > 580-585

Thiazolidinediones have been shown to activate AMP-activated protein kinase activity in cultured cells. Whether they have a similar effect in vivo and if so whether it is physiologically relevant is not known. To assess these questions, we examined the effects of pioglitazone, administered orally to intact rats, on AMPK phosphorylation (AMPK-P) (a measure of its activation) and acetyl CoA carboxylase...

article

Malonyl-CoA and AMP-activated protein kinase: An expanding partnership

Asish K. Saha, Neil B. Ruderman

Molecular and Cellular Biochemistry > 2003 > 253 > 1-2 > 65-70

Insulin resistance in skeletal muscle is present in humans with type 2 diabetes (noninsulin-dependent diabetes mellitus) and obesity and in rodents with these disorders. Malonyl CoA is a regulator of carnitine palmitoyl transferase I (CPT I), the enzyme that controls the transfer of long chain fatty acyl CoA into mitochondria where it is oxidized. In rat skeletal muscle, the formation of malonyl CoA...

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Search results for: Asish K. Saha

Lipopolysaccharide (LPS)-induced septic shock causes profound changes in myocardial energy metabolites in pigs

The effects of troglitazone on AMPK in HepG2 cells

Insulin inhibits AMPK activity and phosphorylates AMPK Ser485/491 through Akt in hepatocytes, myotubes and incubated rat skeletal muscle

Estradiol Regulates Brown Adipose Tissue Thermogenesis via Hypothalamic AMPK

SIRT4 Coordinates the Balance between Lipid Synthesis and Catabolism by Repressing Malonyl CoA Decarboxylase

The evolution of insulin resistance in muscle of the glucose infused rat

Ablation of ARNT/HIF1β in Liver Alters Gluconeogenesis, Lipogenic Gene Expression, and Serum Ketones

Ablation of ARNT/HIF1β in Liver Alters Gluconeogenesis, Lipogenic Gene Expression, and Serum Ketones

Concurrent regulation of AMP-activated protein kinase and SIRT1 in mammalian cells

The AMPK-Malonyl-CoA-CPT1 Axis in the Control of Hypothalamic Neuronal Function—Reply

Hypothalamic Fatty Acid Metabolism Mediates the Orexigenic Action of Ghrelin

AMPK regulation of the growth of cultured human keratinocytes

Malonyl-CoA decarboxylase is present in the cytosolic, mitochondrial and peroxisomal compartments of rat hepatocytes

AMPK, the metabolic syndrome and cancer

Dietary polyunsaturated fatty acids enhance hepatic AMP-activated protein kinase activity in rats

AMP-activated protein kinase activators can inhibit the growth of prostate cancer cells by multiple mechanisms

AMPK activity is diminished in tissues of IL-6 knockout mice: the effect of exercise

Pioglitazone treatment activates AMP-activated protein kinase in rat liver and adipose tissue in vivo

Malonyl-CoA and AMP-activated protein kinase: An expanding partnership

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