Search results for: Nicholas A. Cellar

Items from 1 to 6 out of 6 results

article

A new sample preparation and separation combination for precise, accurate, rapid, and simultaneous determination of vitamins B1, B2, B3, B5, B6, B7, and B9 in infant formula and related nutritionals by LC-MS/MS

Nicholas A. Cellar, Sean C. McClure, Louis M. Salvati, Todime M. Reddy

Analytica Chimica Acta > 2016 > 934 > C > 180-185

An improved method was developed for simultaneous determination of the fortified forms of thiamine (B₁), riboflavin (B₂), nicotinamide and nicotinic acid (B₃), pantothenic acid (B₅), pyridoxine (B₆), biotin (B₇), and folic acid (B₉) in infant formulas and related nutritionals. The method employed a simple, effective, and rapid sample preparation followed by liquid chromatography tandem mass spectrometry...

article

Immunodepletion of high abundance proteins coupled on-line with reversed-phase liquid chromatography: A two-dimensional LC sample enrichment and fractionation technique for mammalian proteomics

Nicholas A. Cellar, Anton S. Karnoup, David R. Albers, Marsha L. Langhorst, more

Journal of Chromatography B > 2009 > 877 > 1-2 > 79-85

Proteomic analysis can be hampered by the large concentration distribution of proteins. Immunoaffinity techniques have been applied to selectively remove high abundant proteins (HAP's) from samples prior to analysis. Although immunodepletion of HAP's has been shown to enable greater detection of low abundance proteins, the resulting fractions are often diluted 5–10-fold during the process. Various...

article

Cross species applicability of abundant protein depletion columns for ribulose-1,5-bisphosphate carboxylase/oxygenase

Nicholas A. Cellar, Krishnamoorthy Kuppannan, Marsha L. Langhorst, Weiting Ni, more

Journal of Chromatography B > 2008 > 861 > 1 > 29-39

In plants, ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) is an important enzyme in the Calvin cycle, catalyzing the first step of carbon fixation. Because of its critical role in photosynthesis, RuBisCO comprises 30–60% of the total protein content in green leaf tissue and represents a major protein which can interfere with determination of lower abundance proteins in plant proteomics...

article

Microdialysis coupled on-line to capillary liquid chromatography with tandem mass spectrometry for monitoring acetylcholine in vivo

Holly M. Shackman, Minshan Shou, Nicholas A. Cellar, Christopher J. Watson, more

Journal of Neuroscience Methods > 2007 > 159 > 1 > 86-92

Capillary liquid chromatography–mass spectrometry (cLC–MS) was coupled on-line to microdialysis sampling to monitor endogenous acetylcholine (ACh) from the rodent brain. In vivo microdialysate sampled at 0.6μL/min from the striatum of ketamine or chloral hydrate anesthetized rats was loaded onto a sample loop and then injected onto a ∼5cm long strong cation exchange (SCX) capillary column. A step...

article

Microfluidic Chip for Low-Flow Push-Pull Perfusion Sampling in Vivo with On-Line Analysis of Amino Acids

Nicholas A. Cellar, Scott T. Burns, Jens-Christian Meiners, Hao Chen, more

Analytical Chemistry > 2005 > 77 > 21 > 7067 - 7073

article

Discovery and Neurochemical Screening of Peptides in Brain Extracellular Fluid by Chemical Analysis of in Vivo Microdialysis Samples

William E. Haskins, Christopher J. Watson, Nicholas A. Cellar, David H. Powell, more

Analytical Chemistry > 2004 > 76 > 18 > 5523 - 5533

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Keywords

IMMUNODEPLETION (2)
MASS SPECTROMETRY (2)
PROTEOMICS (2)
2DLC (1)
ABUNDANT PROTEIN DEPLETION (1)
ACETYLCHOLINE (1)
ARABIDOPSIS (1)
CANOLA (1)
CAPILLARY LIQUID CHROMATOGRAPHY (1)
CHLORAL HYDRATE (1)
CORN (1)
DEPLETION (1)
INFANT FORMULA (1)
K+ STIMULATION (1)
KETAMINE (1)
LC-MS/MS (1)
LIQUID CHROMATOGRAPHY (1)
MICRODIALYSIS (1)
MULTIDIMENSIONAL CHROMATOGRAPHY (1)
PEPTIDE MASS FINGERPRINT (1)
PROTEIN (1)
RIBULOSE-1,5-BISPHOSPHATE CARBOXYLASE/OXYGENASE (1)
RUBISCO (1)
STRIATUM (1)
STRONG CATION EXCHANGE (1)
TOBACCO (1)
WATER SOLUBLE VITAMINS (1)
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Elsevier (4)
ACS (2)

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Search results for: Nicholas A. Cellar

A new sample preparation and separation combination for precise, accurate, rapid, and simultaneous determination of vitamins B1, B2, B3, B5, B6, B7, and B9 in infant formula and related nutritionals by LC-MS/MS

Immunodepletion of high abundance proteins coupled on-line with reversed-phase liquid chromatography: A two-dimensional LC sample enrichment and fractionation technique for mammalian proteomics

Cross species applicability of abundant protein depletion columns for ribulose-1,5-bisphosphate carboxylase/oxygenase

Microdialysis coupled on-line to capillary liquid chromatography with tandem mass spectrometry for monitoring acetylcholine in vivo

Microfluidic Chip for Low-Flow Push-Pull Perfusion Sampling in Vivo with On-Line Analysis of Amino Acids

Discovery and Neurochemical Screening of Peptides in Brain Extracellular Fluid by Chemical Analysis of in Vivo Microdialysis Samples

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