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Pure cardiolipin deposit onto electrodes is optimized and shown to yield an efficient supported lipid film for promoting cytochrome-c immobilization and electroactivity. Cyclic voltammetry and electrochemical impedance spectroscopy measurements in an aqueous electrolyte with potassium ferri- and ferrocyanide as a redox probe evidence that an optimized pure cardiolipin film is reached for a 7μgcm−2...