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The hypervariable D3 domain of Salmonella flagellin, composed of residues 190–283, is situated at the outer surface of flagellar filaments. A flagellin mutant deprived of the complete D3 domain (ΔD3_FliC) exhibited a significantly decreased thermal stability (T m 41.9 °C) as compared to intact flagellin (T m 47.3 °C). However, the stability of filaments formed from ΔD3_FliC subunits...
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