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Stable isotopes are ideal labels for studying biological processes because they have little or no effect on the biochemical properties of target molecules. The NanoSIMS is a tool that can image the distribution of stable isotope labels with up to 50nm spatial resolution and with good quantitation. This combination of features has enabled several groups to undertake significant experiments on biological...
The existence of genetically altered mice provides the opportunity to elucidate the precise mechanisms of chylomicron remnant removal by the liver. Molecules potentially involved in this process are apoE, the low density lipoprotein (LDL) receptor (R) and the LDL-R related protein (LRP). Single pass perfusion of isolated livers from mice was carried out. In successful perfusions, PO 2 decreased...
Previously, we reported on the determination of 4-hydroxyalkenals as pentafluorobenzyl oxime derivatives by gas chromatography-mass spectrometry. In this study, an improved method is presented which allows quantitative detection of 4-hydroxyalkenals in tissues by using two stable isotope-labeled internal standards, 9D 3 -4-hydroxynonenal and 9D 3 -4-hydroxyhexenal. This assay was used...
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