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This paper describes a hydrogel microfiber encapsulating bacteria using an axisymmetric microfluidic device that creates co-axial laminar flow. Since the encapsulated bacteria proliferate at high density in the core of the microfiber, they easily form biofilms; these films are suggested to be important for the cell viability and high-level bacterial secretion. Here, we succeeded in preparing densely...
This paper describes a centimeter-scale living cell fabric made of cell-containing core-shell hydrogel fibers, “cell fiber.” We improved core-shell fiber applicable to various types of cells, and precisely characterized their biofunctions and mechanical properties. Using these cell fibers, we demonstrate a centimeter-scale living cell fabric woven by our micro weaving machine. We believe that our...
This paper describes a dynamic microarray system for pairing adherent cells at single cell level. By introducing single cells cultured on mobile Parylene microplates into the system, we achieved pairing of single cells maintaining their physiological properties. By taking advantage of processibility of Parylene, we changed the shape and fabricated microgrooves on the plate surface. These modifications...
This paper describes an integrated bio-reaction platform composed of silicon nanotweezers and open microfluidics for real time biomechanical assays. The silicon nanotweezers can sense slight biological modification of the trapped sample due to stable frequency response with high Q factor in liquid. The microfluidic device integrates active valves for controlling the biological medium. Biomolecular...
As the main component of cellular membranes, lipid bilayers were widely investigated. A prerequisite approach may consist in studying an artificial bi-layer, which is able to mimic some aspects of a natural membrane through its constituents. Methods were proposed to produce these artificial membranes using different biochip technologies.
We developed a method to prepare size-and shape-controlled islet-cell spheroids. We cultured beta-cell line (MIN6-m9) in PDMS-microwells and enabled to construct their spheroids with the various sizes corresponding to those of the microwells. We demonstrated the geometric analysis of insulin secretion of the cells in the spheroids. We found that these spheroids respond to glucose and subsequently...
We successfully established a three-dimensional (3D) hierarchic cell co-culture system using micro-collagen beads. Our reconstructed tissues have double cell-layers that provide an in vivo-like micro-environment. To prepare the hierarchic tissue structures, we used an axisymmetric flow-focusing device (AFFD) that allows us to encapsulate HepG2 cells within monodisperse collagen beads. We then seeded...
In this study we fabricate a cell-guide on a thin disc-shaped probe with numerous microholes to be implanted between the severed stumps of axons; the cell-guide directs the regenerating axons though the holes in our probe (Fig. 1(a)). Since cell growth is greatly influenced by the topography of the substrate [1-3] we design a SU8 microfluidic cell-guide with a particular shape (Fig. 1(b)) to prevent...
This paper describes a method for handling a single adherent cell using a self-assembly technique. We produce microplates using Parylene with both hydrophilic and hydrophobic sides. We culture the cells on the hydrophilic side, and utilize the hydrophobic side to self-assemble the plates onto the hydrophobic regions. Culturing a single cell on each Parylene microplate facilitates their handling since...
This study describes a membrane-protein based odorant sensor device consisting of microfluidic channels and frog eggs (Xenopus laevis oocyte) expressing olfactory receptors. These receptors are from silkmoth's receptors, BmOR1 and BmOR3 excited by specific pheromones, bombykol and bombykal, respectively. We employ a conventional two electrode voltage clamp method for the signal measurement of the...
This paper describes a simple reusable device that hydrodynamically traps a large number of beads in an array. The device allows us to release the trapped beads by simply reversing a flow direction. The trap and reset operations are extremely simple, robust and highly efficient. With the device, we succeeded in arraying 1000 microbeads, and subsequently releasing them in a few minutes. As the demonstration...
This paper describes single-cell-mounted mobile microplates. Microfabricated SiO2 microplates (20-75 mum in diameter and 2 mum in thickness) carry single adherent cells. The microplates are released freely from a plate-holding PDMS substrate. These microplates enabled us to handle adherent cells as if they were floating cells. We applied this cell-handling technique to two types of assembly: (i) microplate-guided...
We present a method to form monodisperse microcages encapsulating cells with poly-L-lysine (PLL) membrane. These microcages were prepared with a monolithic three-dimensional microfluidic axisymmetric flow-focusing device (3D AFFD) using internal gelation method. The production process of microcage is mild enough for cells. The microcages were sufficiently monodisperse and robust to be trapped in a...
This work is motivated by the need for accurate positioning of both adherent and non-adherent cells into arrays that can be integrated with microfluidic channels in high-throughput screening (HTS) for drug discovery. We describe a microfluidic device that allows rapid production of uniform micro hydrogel capsules that can contain cells. Up to 1times104 hydrogel capsules can be simply formed and arrayed...
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