Search results for: Yanjie Yi

Items from 1 to 14 out of 14 results

article

Study on sampling scheme for detecting mycotoxin during wheat storage

Yanjie Yi, Kun Fan, Youtian Shan, Qianzhen Fu, more

Journal of the Science of Food and Agriculture > 102 > 11 > 4752 - 4758

BACKGROUND Mycotoxin produced by mould is one of the most serious contamination sources in food security. Safe storage of grain has become more important to control food security. Currently, there is no officially approved or standardized sampling scheme for detecting mycotoxin in grain storage worldwide. RESULTS In this study, deoxynivalenol (DON) was taken as a typical mycotoxin in stored wheat...

article

Effects of N‐terminal modifications on the stability of antimicrobial peptide SAMP‐A4 analogues against protease degradation

Ruifang Li, Songlin He, Kedong Yin, Beibei Zhang, more

Journal of Peptide Science > 27 > 10 > n/a - n/a

Infections with multidrug‐resistant (MDR) pathogens are increasingly concerning for public health. Synthesized antimicrobial peptide A4 (SAMP‐A4), a peptide computationally designed by our research team, is a potential drug candidate. However, the antimicrobial peptide SAMP‐A4 is easily degraded in serum. To obtain SAMP‐A4 analogues with high biostability, chemical modifications at its N‐terminus,...

article

Primary research on sampling scheme for analyzing mycotoxin distribution in wheat and rice fields

Yanjie Yi, Kun Fan, Jiaqi Wang, Qianzhen Fu, more

Journal of the Science of Food and Agriculture > 101 > 12 > 4980 - 4986

BACKGROUND Mycotoxins are among the most severe food contaminants. Deoxynivalenol and aflatoxin contamination are predominant in wheat and rice, respectively. Nowadays, there are no standardized and approved grain‐sampling schemes worldwide. This study aimed to develop a scientific grain‐sampling scheme to investigate the regularity of mycotoxin distributed in wheat and rice fields. The data were...

article

Synthesis of tumor-targeted folate conjugated fluorescent magnetic albumin nanoparticles for enhanced intracellular dual-modal imaging into human brain tumor cells

Xueqin Wang, Miaomiao Tu, Baoming Tian, Yanjie Yi, more

Analytical Biochemistry > 2016 > 512 > C > 8-17

Superparamagnetic iron oxide nanoparticles (SPIO NPs), utilized as carriers are attractive materials widely applied in biomedical fields, but target-specific SPIO NPs with lower toxicity and excellent biocompatibility are still lacking for intracellular visualization in human brain tumor diagnosis and therapy. Herein, bovine serum albumin (BSA) coated superparamagnetic iron oxide, i.e. γ-Fe₂O₃ nanoparticles...

article

Molecular tagging of a new broad-spectrum powdery mildew resistance allele Pm2c in Chinese wheat landrace Niaomai

Hongxing Xu, Yanjie Yi, Pengtao Ma, Yanmin Qie, more

Theoretical and Applied Genetics > 2015 > 128 > 10 > 2077-2084

Key message A new broad-spectrum powdery mildew resistance allele Pm2c was identified and mapped in Chinese wheat landrace Niaomai. Abstract Chinese wheat landrace Niaomai showed resistance to 27 of 28 Chinese Blumeria graminis f. sp tritici (Bgt) races. Genetic analysis of an F₂ population and its derived F_2:3 families from the cross Niaomai × Mingxian 169 and backcross population, Niaomai/2*Mingxian...

chapter

Optimization of Medium and Fermentation Conditions for the Antagonistic Bacillus subtilis BS501a Strain Using Response Surface Methodology

Ruifang Li, Yangyuan Tian, Yanjie Yi, Changfu Zhang, more

2011 5th International Conference on Bioinformatics and Biomedical Engineering > 1 - 6

2011 5th International Conference on Bioinformatics and Biomedical Engineering (iCBBE)

article

Constrained use of CCR5 on CD4+ lymphocytes by R5X4 HIV-1: Efficiency of Env–CCR5 interactions and low CCR5 expression determine a range of restricted CCR5-mediated entry

Lamorris M. Loftin, Martha F. Kienzle, Yanjie Yi, Benhur Lee, more

Virology > 2010 > 402 > 1 > 135-148

R5X4 HIV-1 has impaired utilization of CCR5 on primary CD4+ lymphocytes but the mechanisms responsible are not well defined. Using a panel of diverse R5X4 Envs we identified a spectrum of CCR5 use on CD4+ lymphocytes. Greater lymphocyte CCR5 use correlated with relative resistance to CCR5 mAbs and small molecule antagonists. Increasing CCR5 expression on lymphocytes increased the proportion of entry...

article

Chemokine receptor utilization and macrophage signaling by human immunodeficiency virus type 1 gp120: Implications for neuropathogenesis

Yanjie Yi, ChuHee Lee, Qing-Hua Liu, Bruce D. Freedman, more

Journal of NeuroVirology > 2004 > 10 > 1 > 91-96

Human immunodeficiency virus type 1 (HIV-1) uses the chemokine receptors CCR5 and CXCR4 for entry. Macrophages and microglia (M/M) are the principal productively infected brain cells in HIV encephalopathy (HIVE), and neuronal injury is believed to result both from direct effects of viral proteins and indirect effects mediated by macrophage activation and secretion of neurotoxic products. In vitro,...

article

An unusual syncytia-inducing human immunodeficiency virus type 1 primary isolate from the central nervous system that is restricted to CXCR4, replicates efficiently in macrophages, and induces neuronal apoptosis

Yanjie Yi, Wei Chen, Ian Frank, Joann Cutilli, more

Journal of NeuroVirology > 2003 > 9 > 4 > 432-441

Macrophage/microglia cells are the principal targets for human immunodeficiency virus type 1 (HIV-1) in the central nervous system (CNS). Prototype HIV-1 isolates from the CNS are macrophage (M)-tropic, non-syncytia-inducing (NSI), and use CCR5 for entry (R5 strains), but whether syncytia-inducing (SI) CXCR4-using X4 strains might play a role in macrophage/microglia infection and neuronal injury is...

article

A CCR5/CXCR4-Independent Coreceptor Pathway on Human Macrophages Supports Efficient SIV Env-Mediated Fusion but Not Infection: Implications for Alternative Pathways of Viral Entry

Yanjie Yi, Anjali Singh, Stuart N. Isaacs, Ronald G. Collman

Virology > 2001 > 284 > 1 > 142-151

Several coreceptors in addition to CCR5 and CXCR4 support immunodeficiency virus entry in transfected cells, but whether they could play a role in HIV-1 pathogenesis is uncertain. To probe whether human macrophages express potentially functional alternative entry pathways, we analyzed cell–cell fusion and infection of primary macrophage by several SIVmac Envs. All Envs fused with normal macrophages...

article

Coreceptor Choice and T Cell Depletion by R5, X4, and R5X4 HIV-1 Variants in CCR5-Deficient (CCR5Δ32) and Normal Human Lymphoid Tissue

Nina Malkevitch, David H. McDermott, Yanjie Yi, Jean-Charles Grivel, more

Virology > 2001 > 281 > 2 > 239-247

Coreceptor utilization by HIV-1 is an important determinant of pathogenesis. However, coreceptor selectivity is defined in vitro, while in vivo critical pathogenic events occur in lymphoid tissues. Using pharmacological inhibitors, we recently provided evidence that coreceptor selectivity by the R5X4 dual-tropic isolate 89.6 was more restricted in ex vivo infected lymphoid tissue than in vitro [S...

article

A macrophage fusion assay for rapid screening of cloned HIV-1 Env using dual recombinant vaccinia viruses expressing distinct RNA polymerases

Stuart N. Isaacs, Yanjie Yi, Anjali Singh, Ronald G. Collman

Journal of Virological Methods > 1999 > 81 > 1-2 > 55 - 61

HIV-1 cell tropism is determined initially at the level of fusion mediated by the viral envelope glycoprotein (Env). Cell-cell fusion assays are employed widely to study Env-mediated fusion, and generally require transfection of target cells with a reporter plasmid that is activated upon fusion with Env-expressing effector cells. Macrophages are an important target for HIV-1, but fusion studies using...

article

Regions in β-Chemokine Receptors CCR5 and CCR2b That Determine HIV-1 Cofactor Specificity

Joseph Rucker, Michel Samson, Benjamin J. Doranz, Frédérick Libert, more

Cell > 1996 > 87 > 3 > 437-446

Macrophage-tropic (M-tropic) HIV-1 strains use the β-chemokine receptor CCR5, but not CCR2b, as a cofactor for membrane fusion and infection, while the dual-tropic strain 89.6 uses both. CCR5/2b chimeras and mutants were used to map regions of CCR5 important for cofactor function and specificity. M-tropic strains required either the amino-terminal domain or the first extracellular loop of CCR5. A...

article

A Dual-Tropic Primary HIV-1 Isolate That Uses Fusin and the β-Chemokine Receptors CKR-5, CKR-3, and CKR-2b as Fusion Cofactors

Benjamin J. Doranz, Joseph Rucker, Yanjie Yi, Robert J. Smyth, more

Cell > 1996 > 85 > 7 > 1149-1158

Here, we show that the β-chemokine receptor CKR-5 serves as a cofactor for M-tropic HIV viruses. Expression of CKR-5 with CD4 enables nonpermissive cells to form syncytia with cells expressing M-tropic, but not T-tropic, HIV-1 env proteins. Expression of CKR-5 and CD4 enables entry of a M-tropic, but not a T-tropic, virus strain. A dual-tropic primary HIV-1 isolate (89.6) utilizes both Fusin and CKR-5...

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