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Purpose: The aim was to measure concentration changes of ions in a single cell type induced by heat shock (HS) or ethanol.Methods: C6 glioma cells were grown on polystyrol beads. Intracellular H + , Na + , K + and membrane potential were determined by means of continuous 31 P - and 23 Na -nuclear magnetic resonance (NMR), ion sensitive fluorescent...
Purpose: The objective of our investigations was to monitor heat-shock-induced intracellular sodium activity in glia cells applying the sodium-sensitive probe SBFI.Methods: Cells were grown on polystyrol- or gelatinebeads. These populated beads were filled in a perfused and heated quartz cuvette of a fluorescence spectrometer. The incubation with SBFI-AM was done inside the spectrometer.Results: A...
Purpose: The objective of the investigation was to study intracellular volume, apparent water diffusion constants and restricted diffusion of Chlorella fusca cells under different environmental conditions.Methods: Chlorella cells were immobilized allowing to perfuse them with nutrient. By optional illumination the cells can be measured under photosynthetic conditions. A Bruker AMX360 system with an...
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