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The actin and the tubulin cytoskeleton organization during the differentiation of the embryo-suspensor in Alisma plantago-aquatica was studied in comparison with the development of embryo, using immunofluorescence detection and rhodamine-phalloidin assay. At the early stage of the suspensor basal cell development (from 2- to ~10-celled embryos) microfilaments form an abundant network in the cytoplasm...
We examined the development of the endosperm chalazal haustorium of Rhinanthus serotinus, using histochemical assays and light and electron microscopy. The chalazal haustorium is a huge single cell containing two enlarged nuclei. The nuclei are located in the middle of the haustorium cell. At the chalazal end of the haustorium cell structure, ultrastructural study revealed the presence of a transfer...
The study used Actinidia deliciosa endosperm-derived callus to investigate aspects of the morphology, histology and chemistry of extracellular matrix (ECM) structures in morphogenically stable tissue from long-term culture. SEM showed ECM as a membranous layer or reticulated fibrillar and granular structure linking the peripheral cells of callus domains. TEM confirmed that ECM is a distinct heterogeneous...
Podział mejotyczny mikrosporocytów i rozwój ziarna pyłku Gagea lutea (L.) Ker.-Gaw. (Liliaceae) obserwowano w mikroskopie fluorescencyjnym (światło wzbu- dzające 400 nm), po rozgnieceniu pylników w roztworze DAPI (fluorochrom barwią- cy DNA). W 70% mikrosporocytów i ziaren pyłku mikrosporogeneza i rozwoj ziarna pyłku przebiega w regularny sposób. W pozostałych mikrosporocytach i ziarnach pyłku (30%)...
The paper reports a study of karyological differentiation of the chalazal endosperm haustorium of Rhinanthus serotinus (Schönheit) Oborny. Polyploidization began soon after the formation of the haustorium cell. The fully differentiated chalazal haustorium is a large elongated cell containing two huge nuclei. The nuclei are situated halfway along the length of the haustorium cell. Measurements of their...
The actin cytoskeleton of endosperm and of the mature endosperm chalazal haustorium cell of Rhinanthus serotinus was examined by immunohistochemistry and epifluorescence microscopy. A prominent actin cytoskeleton composed of numerous cross-linked filaments is present at the distal pole of the chalazal haustorium cell. Thick, longitudinally oriented bundles of microfilaments localize in transvacuolar...
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