Search results for: Wanhui Liu

Items from 1 to 20 out of 42 results

article

Knockout of a highly GC‐rich gene in Burkholderia pyrrocinia by recombineering with freeze‐thawing transformation

Feifei Chen, Jianren Ye, Wanhui Liu, Chonlong Chio, more

Molecular Plant Pathology > 22 > 7 > 843 - 857

Genetic transformation is a valuable and essential method that provides powerful insights into the gene function of microorganisms and contributes to the construction of engineered bacteria. Here, we developed a novel genetic transformation system to easily knock out a highly GC‐rich gene (74.71% GC) from Burkholderia pyrrocinia JK‐SH007, a biocontrol strain of poplar canker disease. This system revealed...

article

Liquid chromatography tandem mass spectrometry method for determination of fulvestrant in rat plasma and its application to pharmacokinetic studies of a novel fulvestrant microcrystal

Guangyi Leng, Yanhua Zuo, Jiahui Hu, Fei Yu, more

Biomedical Chromatography > 34 > 10 > n/a - n/a

Fulvestrant (‘Faslodex’), an estrogen receptor antagonist, is available for the treatment of advanced breast cancer. The oil‐based vehicle of Faslodex can lead to various adverse effects. A novel fulvestrant microcrystal (aqueous suspension) was developed in this study to eliminate these adverse effects. A sensitive and robust liquid chromatography tandem mass spectrometry method was developed and...

article

A Systematic Study on the Dynamic Softening Behavior of a Heat-Resistant Alloy Considering Thermal and Microstructural Effects

Weicheng Jiang, Li Ruihong, Fengzhi Wu, Ye Cheng, more

Journal of Materials Engineering and Performance > 2019 > 28 > 1 > 308-320

Deep understanding of the relationships among flow softening behaviors, microstructure evolution and process parameters is an essential issue due to the varied strain rate and temperature in industrial hot working schedules. In this research, substantial hot compression tests of a SNCrW heat-resistant alloy were conducted to quantitatively investigate the extrinsic dynamic flow softening behaviors...

article

Numerical and experimental study on the influence of material characteristics on the levitation performance of squeeze-film air bearing

Minghui Shi, Lin An, Kai Feng, Zhiyang Guo, more

Tribology International > 2018 > 126 > C > 307-316

This research investigated the effects of material characteristics on the load-carrying capacity of squeeze-film air bearings (SFABs). Bearings with the same size and structure but are made of different materials, namely, aluminum (AL 2024), brass (CuSn35), and spring steel (60Si2Mn), were manufactured and tested. A theoretical analysis model was established to investigate the levitation performance...

article

Nonlinear dynamic analysis of a rotor-bearing system with porous tilting pad bearing support

Yihua Wu, Kai Feng, Yun Zhang, Wanhui Liu, more

Nonlinear Dynamics > 2018 > 94 > 2 > 1391-1408

Porous tilting pad bearings (PTPBs) show a potential for using in high-speed, high precision rotating machinery that requires high bearing stiffness and good stability. In this study, the nonlinear characteristics of a rotor supported on PTPBs are investigated. The Darcy equations and the modified Reynolds equations are adopted to establish the gas flow model in porous materials and gas film region,...

article

Constituents of Mediterranean Spices Counteracting Vascular Smooth Muscle Cell Proliferation: Identification and Characterization of Rosmarinic Acid Methyl Ester as a Novel Inhibitor

Rongxia Liu, Elke H. Heiss, Birgit Waltenberger, Tina Blažević, more

Molecular Nutrition & Food Research > 62 > 7 > n/a - n/a

Scope Aberrant vascular smooth muscle cell (VSMC) proliferation is involved in atherosclerotic plaque formation and restenosis. Mediterranean spices have been reported to confer cardioprotection, but their direct influence on VSMCs has largely not been investigated. This study aims at examining rosmarinic acid (RA) and 11 related constituents for inhibition of VSMC proliferation in vitro, and at...

article

Validated LC–MS/MS method for the simultaneous determination of rotigotine and its prodrug in rat plasma and an application to pharmacokinetics and biological conversion in vitro

Chunjie Sha, Jiangbin Han, Fengjuan Zhao, Xin Shao, more

Journal of Pharmaceutical and Biomedical Analysis > 2017 > 146 > C > 24-28

Rotigotine behenate (RGTB), a long chain alkyl ester of the prodrug of rotigotine (RGT), has been synthesized for use in a sustained delivery system. The aim of the present report was to develop and validate a simple, sensitive and reliable LC–MS/MS method for the simultaneous determination of RGT and its prodrug RGTB in rat plasma samples. Detection was performed on a 1290 Infinity UPLC coupled Triple...

article

Bifurcation and nonlinear dynamic behaviours of a metal mesh damped flexible pivot tilting pad gas bearing system

Wanhui Liu, Kai Feng, Peng Lyu

Nonlinear Dynamics > 2018 > 91 > 1 > 655-677

The nonlinear dynamics of a rigid rotor supported on two identical metal mesh damped flexible pivot tilting pad gas bearings is investigated. This new type gas bearing is used because of high effective damping level and adequate compliance with variations in rotor geometry or misalignment. Time domain simulation that couples rotor motion equations, unsteady Reynolds equation and pad motion (considering...

article

Spirotriphenylamine based star-shaped D-A molecules meeting AIE chromophore for both efficient solution-processed doped and nondoped blue organic light-emitting diodes

Yafei Wang, Wanhui Liu, Zuoming Qu, Hua Tan, more

Dyes and Pigments > 2017 > 143 > C > 173-182

To explore the structure-property of donor-acceptor (D-A) type blue emitter, in this contribution, four star-shaped compounds of p-TPA, m-TPA, p-TPA-TPE and m-TPA-TPE using spirotriphenylamine as the donor unit and triazine as the acceptor core were designed and prepared. The tetraphenylethene (TPE) unit was introduced into the D-A molecules to enhance the emission efficiency in solid state. The photophysical...

article

Analysis and experimental study on a novel gas foil bearing with nested compression springs

Kai Feng, Wanhui Liu, Rui Yu, Zhiming Zhang

Tribology International > 2017 > 107 > C > 65-76

This paper presents a theoretical analysis and experimental study on a novel nested compression spring gas foil bearing (NSFB), which uses a layer of springs as elastic support structure. The stiffness and damping of the support structure can be easily controlled by adjusting the spring number and diameter. Reducing the stiffness of the springs at both ends of the NSFB significantly improves bearing...

article

Effect of microfabrication defects on the performance of rarefaction gas-lubricated micro flexure pivot tilting pad gas bearing in power MEMS

Wenjun Li, Wanhui Liu, Kai Feng

Microsystem Technologies > 2017 > 23 > 8 > 3401-3419

The performances of a micro flexure pivot tilting pad gas bearing with the combined effect of rarefaction and the manufacturing defects were studied. A slip model derived from kinetic theory was used to simulate the flow behavior of the micro bearing. The gas film thickness equation was modeled to express the effects of microfabrication defects. The static, dynamic and nonlinear characteristics of...

article

Preparation and evaluation of rotigotine-loaded implant for the treatment of Parkinson’s disease and its evolution study

Aiping Wang, Yanxiang Liu, Rongcai Liang, Xuemei Zhang, more

Saudi Pharmaceutical Journal > 2016 > 24 > 3 > 363-370

To develop rotigotine-loaded implants (RI) to achieve continuous release of rotigotine for long duration for the treatment of Parkinson’s disease (PD).RI was prepared by hot-melt extrusion method using poly (lactide-co-glycolide) (PLGA) as the matrix. In vitro drug release was optimized by drug loading, melting temperature during preparing process and additives. The surface and internal morphology...

article

Synthesis of a bi-functional dendrimer-based nanovehicle co-modified with RGDyC and TAT peptides for neovascular targeting and penetration

Jingjing Li, Xuemei Zhang, Meng Wang, Xuejuan Li, more

International Journal of Pharmaceutics > 2016 > 501 > 1-2 > 112-123

The dual-ligand dendritic polyamidoamine-(polyethylene glycol)_n-cyclic RGDyC peptide-(TAT peptide) (PP_nR(T)) with various supplied molar ratios of polyethylene glycol (PEG) to polyamidoamine (PAMAM) (n=5, 15, 30) were designed as drug-carriers for the treatment of neovascular diseases; their targeting and penetrating effects were subsequently evaluated. ¹H NMR demonstrated PP_nR(T) was successfully...

article

Development and validation of a micellar electrokinetic capillary chromatography method for the determination of goserelin and related substances

Feifei Gong, Huaxin Yang, Wen Sun, Junzi Cao, more

ELECTROPHORESIS > 37 > 4 > 623 - 629

An MEKC method for the analysis of goserelin and related substances has been developed using a combination of additives including CTAB, β‐CD, and sodium hexanesulfonate. For this assay, the running buffer (pH and additives) and separation conditions (voltage and temperature) were optimized. The optimized system was the following: 200 mM 6‐aminocaproic acid buffer (pH 4.2) supplemented with 175 mM...

article

Theoretical model of flexure pivot tilting pad gas bearings with metal mesh dampers in parallel

Kai Feng, Wanhui Liu, Zhiming Zhang, Tong Zhang

Tribology International > 2016 > 94 > Complete > 26-38

The application of flexure pivot tilting pad gas bearings (FPTPGBs) with metal mesh dampers (MMDs) in parallel in high-performance oil-free turbomachineries has been considered because of their high effective damping level and adequate compliance to the variations in rotor geometry. This study proposes a comprehensive theoretical model of these bearings by coupling the FPTPGB model and MMD model....

article

Stereoselective Formation and Metabolism of 20(S)‐Protopanaxadiol Ocotillol Type Epimers in Vivo and in Vitro

Wenyan Wang, Li Wang, Xiangmeng Wu, Lixiao Xu, more

Chirality > 27 > 2 > 170 - 176

(20S,24S)‐epoxy‐dammarane‐3,12,25‐triol (24S‐epimer) and (20S,24R)‐epoxy‐ dammarane‐3,12,25‐triol (24R‐epimer), a pair of ocotillol type epimers, were identified as the main metabolites of 20(S)‐protopanaxadiol (PPD). The aim of this study was to systematically investigate the formation and metabolism of this pair of epimers in vivo and in vitro and to elucidate the isoforms of cytochrome P450 enzymes...

article

The Glucose-Lowering Potential of Exenatide Delivered Orally via Goblet Cell- Targeting Nanoparticles

Xiang Li, Chenhui Wang, Rongcai Liang, Fengying Sun, more

Pharmaceutical Research > 2015 > 32 > 3 > 1017-1027

Purpose Exenatide, a potent insulinotropic agent, can be used for the treatment of non-insulin-dependent diabetes mellitus. However, the need for frequent injections seriously limits its therapeutic utility. The aim of present report was to develop an orally available exenatide formulation using goblet cell-targeting nanoparticles (NPs). Method The exenatide-loaded nanoparticles were prepared...

article

Development of andrographolide loaded PLGA microspheres: Optimization, characterization and in vitro–in vivo correlation

Yunxia Jiang, Fang Wang, Hui Xu, Hui Liu, more

International Journal of Pharmaceutics > 2014 > 475 > 1-2 > 475-484

The purpose of this study was to develop a sustained-release drug delivery system based on the injectable PLGA microspheres loaded with andrographolide. The andrographolide loaded PLGA microspheres were prepared by emulsion solvent evaporation method with optimization of formulation using response surface methodology (RSM). Physicochemical characterization, in vitro release behavior and in vivo pharmacokinetics...

article

An LC–MS/MS method for the simultaneous determination of goserelin and testosterone in rat plasma for pharmacokinetic and pharmacodynamic studies

Shu Zhang, Jiangbin Han, Guangyi Leng, Xin Di, more

Journal of Chromatography B > 2014 > 965 > Complete > 183-189

A liquid chromatography–electrospray ionization tandem mass spectrometry (LC–ESI-MS/MS) method was developed, using testosterone-d ₃ as a surrogate analyte, for the simultaneous quantification of goserelin and testosterone in rat plasma. According to this method, the pharmacokinetic and pharmacodynamic data were obtained from a single plasma sample aliquot. The method involved the addition...

article

Charge profiling and stability testing of biosimilar by capillary isoelectric focusing

Junzi Cao, Wen Sun, Feifei Gong, Wanhui Liu

ELECTROPHORESIS > 35 > 10 > 1461 - 1468

CIEF was developed for the rapid analysis of charge heterogeneity of trastuzumab biosimilar using commercially available fluorocarbon‐coated capillary. The CIEF master mix was composed of 0.30% w/v methyl cellulose, 2.3 M urea, 56.8 mM l‐arginine, 1.52 mM iminodiacetic acid, 4.5% v/v carrier ampholytes (broad‐range pI 3–10 and narrow‐range pI 8–10.5 with ratio of 3:1), and 0.45% v/v 10.0, 9.5, 7.0,...

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INFONA - science communication portal

Search results for: Wanhui Liu

Knockout of a highly GC‐rich gene in Burkholderia pyrrocinia by recombineering with freeze‐thawing transformation

Liquid chromatography tandem mass spectrometry method for determination of fulvestrant in rat plasma and its application to pharmacokinetic studies of a novel fulvestrant microcrystal

A Systematic Study on the Dynamic Softening Behavior of a Heat-Resistant Alloy Considering Thermal and Microstructural Effects

Numerical and experimental study on the influence of material characteristics on the levitation performance of squeeze-film air bearing

Nonlinear dynamic analysis of a rotor-bearing system with porous tilting pad bearing support

Constituents of Mediterranean Spices Counteracting Vascular Smooth Muscle Cell Proliferation: Identification and Characterization of Rosmarinic Acid Methyl Ester as a Novel Inhibitor

Validated LC–MS/MS method for the simultaneous determination of rotigotine and its prodrug in rat plasma and an application to pharmacokinetics and biological conversion in vitro

Bifurcation and nonlinear dynamic behaviours of a metal mesh damped flexible pivot tilting pad gas bearing system

Spirotriphenylamine based star-shaped D-A molecules meeting AIE chromophore for both efficient solution-processed doped and nondoped blue organic light-emitting diodes

Analysis and experimental study on a novel gas foil bearing with nested compression springs

Effect of microfabrication defects on the performance of rarefaction gas-lubricated micro flexure pivot tilting pad gas bearing in power MEMS

Preparation and evaluation of rotigotine-loaded implant for the treatment of Parkinson’s disease and its evolution study

Synthesis of a bi-functional dendrimer-based nanovehicle co-modified with RGDyC and TAT peptides for neovascular targeting and penetration

Development and validation of a micellar electrokinetic capillary chromatography method for the determination of goserelin and related substances

Theoretical model of flexure pivot tilting pad gas bearings with metal mesh dampers in parallel

Stereoselective Formation and Metabolism of 20(S)‐Protopanaxadiol Ocotillol Type Epimers in Vivo and in Vitro

The Glucose-Lowering Potential of Exenatide Delivered Orally via Goblet Cell- Targeting Nanoparticles

Development of andrographolide loaded PLGA microspheres: Optimization, characterization and in vitro–in vivo correlation

An LC–MS/MS method for the simultaneous determination of goserelin and testosterone in rat plasma for pharmacokinetic and pharmacodynamic studies

Charge profiling and stability testing of biosimilar by capillary isoelectric focusing

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