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A Reverse Transcription Loop‐Mediated Isothermal Amplification (RT‐LAMP) assay was employed to develop a simple and efficient system for the detection of Zucchini yellow mosaic virus (ZYMV) in squash and melon plants. The RT‐LAMP assay took 30 min under isothermal condition at 64°C by employing a set of four primers targeting ZYMV. The sensitivity of RT‐LAMP was 10‐fold greater than that of the RT‐PCR...
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