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Two blue absorbing and emitting mutants (S65G/T203V/E222Q and S65T at pH 5.5) of the green fluorescent protein (GFP) have been investigated through ultrafast time resolved infra-red (TRIR) and fluorescence spectroscopy. In these mutants, in which the excited state proton transfer reaction observed in wild-type GFP has been blocked, the photophysics are dominated by the neutral A state. It was found...
We have experimentally investigated the polarization resolved hyper Rayleigh scattering response from an aqueous suspension of silver nanoparticles the diameter of which is 40nm. In the spectral region, where the harmonic frequency is in resonance with the dipolar mode of the surface plasmon resonance of the particles, the results unambiguously demonstrate that the origin of the response stems from...
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