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Alcohol dehydrogenase (ADH) from solutions of homogenised packed bakers' yeast has been successfully purified using immobilised metal-ion affinity chromatography in an expanded bed. Method scouting carried out using pure ADH solutions loaded onto 5-ml HiTrap columns charged with Zn 2+ , Ni 2+ and Cu 2+ and eluted using 0-50 mM EDTA gradient found that charging...
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