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A newly developed Widefield Surface Plasmon Resonance (WSPR) Microscope was used to investigate the morphology of MG63 bone cells and their interfacial interactions with ECM proteins. This allowed detailed imaging of cell surface coupling at lateral resolution down to ∼ 500 nm. In this work, bone repair was investigated and modulated by different stimulus including growth factors. TGF-β3 is a cytokine...
In this study, the nature of cell attachment and contraction on the surface of a shear sensitive cholesteryl ester liquid crystal (LC) was examined. This interaction has the potential to be utilized as a novel cell force transducing assay. Preliminary studies indicated that cells cultured on the LC induce deformations in the underlying LC layer. This study aimed at determining if those deformations...
Widefield Surface Plasmon Resonance (WSPR) microscope was used to investigate cell surface interactions under two different culture conditions: bone cells cultured on SPR substrate with transforming growth factor β3 (TGF-β3) and without as control. Trypsinisation was carried out in order to investigate its effect on cell detachment, in the presence of TGF-β3, HCl or BSA/HCl solutions. Trypsin was...
This paper describes the use of a PC-based system for acquisition and processing of data recorded from electrical active and excitable cells cultured over microfabricated arrays of field-effect transistors. Using these recording devices, limitations of conventional recording techniques such as those associated with making long-term and multisite recordings can be overcome. This system has been tested...
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