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Four different activated methacrylatic monoliths, acting as pore‐through‐flow membrane reactors in order to reduce enzyme costs and mass‐transfer limitations, were characterized as support for immobilization of β‐galactosidase to produce galacto‐oligosaccharides (GOS). GOS synthesis was studied in different operation modes. A higher affinity of the immobilized enzyme towards the transgalactosylation...
As lactose is an increasing by‐product of dairy industry, the selective conversion to prebiotic galacto‐oligosaccharides (GOSs) by the enzyme β‐galactosidase represents a highly attractive value creation. The synthesis of GOSs is characterized by an equilibrium‐limited reaction, which can be overcome by a coupling with an enzymatic reduction of the side product glucose to achieve an increase in product...
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