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X-ray reflectivity is used to study the interaction of C2 domains of cytosolic phospholipase A2 (cPLA2α-C2) with a Langmuir monolayer of 1-stearoyl-2-oleoyl-sn-glycero-3-phosphocholine (SOPC) supported on a buffered aqueous solution containing Ca2+. The reflectivity is analyzed in terms of the known crystallographic structure of cPLA2α-C2 domains and a slab model representing the lipid layer to yield...
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