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The methylotrophic yeast Pichia pastoris was tested for heterologous expression of the mouse 5-HT 5A receptor. Three different expression plasmids were constructed where the cDNA of the receptor was cloned under the transcriptional control of the highly inducible promotor of the P. pastoris alcohol oxidase 1 (AOX1) gen. The expression plasmids differed with respect to the signal sequences...
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