The eryA gene of the bacterial pathogen Brucella abortus has been functionally expressed in Escherichia coli. The resultant EryA was shown to catalyze the ATP-dependent conversion of erythritol to l-erythritol-4-phosphate (l-E4P). The steady state kinetic parameters of this reaction were determined and the enzyme was used to prepare l-E4P which was shown to be a weak inhibitor of 2-C-methyl-d-erythritol-4-phosphate...
Dimers and trimers of uridylic acid with a phenylalanine residue at their 5'-terminus were prepared in solution via intermediates with acid-labile protecting groups. A 5'-aminoacylated octanucleotide was prepared on solid support using a phosphoramidate linker and an allyloxycarbonyl-protected phenylalanine.
The affinity of amide-linked 5'-aminoacyl and 5'-dipeptidyl DNA octamers for two RNA undecamers with 3'-overhangs was measured via UV melting analysis. A sequence-dependent increase in melting points was observed. At low ionic strength, two appended lysine residues elevate melting points more than two additional A:U base pairs.
5′-Amino-5′-deoxythymidine was prepared from thymidine in two steps and converted to its known 5′-methoxytrityl-protected 3′-phosphoramidite building block for DNA assembly on solid supports. Using this building block, peptide-DNA hybrids were synthesized in stepwise manner or via fragment condensation, both as single compounds and as small combinatorial libraries.
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