Search results for: Arie J. Verkleij

Items from 1 to 20 out of 46 results

article

Optimizing immuno-labeling for correlative fluorescence and electron microscopy on a single specimen

Matthia A. Karreman, Alexandra V. Agronskaia, Elly G. van Donselaar, Karin Vocking, more

Journal of Structural Biology > 2012 > 180 > 2 > 382-386

Correlative fluorescence and electron microscopy has become an indispensible tool for research in cell biology. The integrated Laser and Electron Microscope (iLEM) combines a Fluorescence Microscope (FM) and a Transmission Electron Microscope (TEM) within one set-up. This unique imaging tool allows for rapid identification of a region of interest with the FM, and subsequent high resolution TEM imaging...

article

A biparatopic anti‐EGFR nanobody efficiently inhibits solid tumour growth

Rob C. Roovers, Maria J.W.D. Vosjan, Toon Laeremans, Rachid el Khoulati, more

International Journal of Cancer > 129 > 8 > 2013 - 2024

The epidermal growth factor receptor (EGFR) has been shown to be a valid cancer target for antibody‐based therapy. At present, several anti‐EGFR monoclonal antibodies have been successfully used, such as cetuximab and matuzumab. X‐ray crystallography data show that these antibodies bind to different epitopes on the ecto‐domain of EGFR, providing a rationale for the combined use of these two antibody...

article

Arabidopsis PLETHORA Transcription Factors Control Phyllotaxis

Kalika Prasad, Stephen P. Grigg, Michalis Barkoulas, Ram Kishor Yadav, more

Current Biology > 2011 > 21 > 13 > 1123-1128

The pattern of plant organ initiation at the shoot apical meristem (SAM), termed phyllotaxis, displays regularities that have long intrigued botanists and mathematicians alike. In the SAM, the central zone (CZ) contains a population of stem cells that replenish the surrounding peripheral zone (PZ), where organs are generated in regular patterns. These patterns differ between species and may change...

article

VIS2FIX: A High‐Speed Fixation Method for Immuno‐Electron Microscopy

Matthia A. Karreman, Elly G. van Donselaar, Hans C. Gerritsen, C. Theo Verrips, more

Traffic > 12 > 7 > 806 - 814

Immuno‐transmission electron microscopy (TEM) is the technique of choice for high‐resolution localization of proteins in fixed specimen. Here we introduce 2 novel methods for the fixation of sections from cryo‐immobilized samples that result in excellent ultrastructural preservation. These high‐speed fixation techniques, both called VIS2FIX, allow for a reduction in sample preparation time from at least 1 week to only 8 h. The methods were validated in immuno‐TEM experiments on THP‐1 monocytes, human umbilical vein endothelial cells (HUVECs) and Madin–Darby canine kidney (MDCK‐II) cells. The fixation and retention of neutral lipids is demonstrated, offering unique prospects for the application of immuno‐TEM in the lipidomics field. Furthermore, the VIS2FIX methods were successfully employed in correlative fluorescence and electron microscopy....

article

The making of frozen-hydrated, vitreous lamellas from cells for cryo-electron microscopy

Michael F. Hayles, D.A. Matthijs de Winter, Chris T.W.M. Schneijdenberg, Johannes D. Meeldijk, more

Journal of Structural Biology > 2010 > 172 > 2 > 180-190

There has been a long standing desire to produce thick (up to 500nm) cryo-sections of fully hydrated cells and tissue for high-resolution analysis in their natural state by cryo-transmission electron microscopy. Here, we present a method that can successfully produce sections (lamellas in FIB-SEM terminology) of fully hydrated, unstained cells from high-pressure frozen samples by focused ion beam...

article

Spiral Coating of the Endothelial Caveolar Membranes as Revealed by Electron Tomography and Template Matching

Misjaël N. Lebbink, Nuria Jiménez, Karin Vocking, Liesbeth H. Hekking, more

Traffic > 11 > 1 > 138 - 150

Caveolae are invaginations of the plasma membrane involved in multiple cellular processes, including transcytosis. In this paper we present an extensive 3‐D electron tomographic study of the endothelial caveolar system in situ. Analysis of large cellular volumes of (high‐pressure frozen, freeze‐substituted and epon‐embedded) human umbilical vein endothelial cells (HUVECs) provided a notable view on...

article

Sol−Gel Transitions and Liquid Crystal Phase Transitions in Concentrated Aqueous Suspensions of Colloidal Gibbsite Platelets

Maurice C. D. Mourad, Dmytro V. Byelov, Andrei V. Petukhov, D. A. Matthijs de Winter, more

The Journal of Physical Chemistry B > 2009 > 113 > 34 > 11604 - 11613

article

Quantitative Structural Analysis of Binary Nanocrystal Superlattices by Electron Tomography

Heiner Friedrich, Cedric J. Gommes, Karin Overgaag, Johannes D. Meeldijk, more

Nano Letters > 2009 > 9 > 7 > 2719 - 2724

article

Electron Tomography for Heterogeneous Catalysts and Related Nanostructured Materials

Heiner Friedrich, Petra E. de Jongh, Arie J. Verkleij, Krijn P. de Jong

Chemical Reviews > 2009 > 109 > 5 > 1613 - 1629

article

Septal pore complex morphology in the Agaricomycotina (Basidiomycota) with emphasis on the Cantharellales and Hymenochaetales

Kenneth G.A. van Driel, Bruno M. Humbel, Arie J. Verkleij, Joost Stalpers, more

Mycological Research > 2009 > 113 > 5 > 559-576

The ultrastructure of septa and septum-associated septal pore caps are important taxonomic markers in the Agaricomycotina (Basidiomycota, Fungi). The septal pore caps covering the typical basidiomycetous dolipore septum are divided into three main phenotypically recognized morphotypes: vesicular-tubular (including the vesicular, sacculate, tubular, ampulliform, and globular morphotypes), imperforate,...

article

Induced membrane domains as visualized by electron tomography and template matching

Misjaël N. Lebbink, Elly van Donselaar, Bruno M. Humbel, Louis O. Hertzberger, more

Journal of Structural Biology > 2009 > 166 > 2 > 156-161

Membranes play a crucial role in many cellular processes, and it is therefore not surprising that many electron tomographic studies in life sciences concern membranous structures. While these tomographic studies provide many new insights into membrane connections and continuities in three dimensions, they are mostly limited to a macro-morphological level. In this paper, we demonstrate that by combining...

article

Tannic acid-mediated osmium impregnation after freeze-substitution: A strategy to enhance membrane contrast for electron tomography

Nuria Jiménez, Karin Vocking, Elly G. van Donselaar, Bruno M. Humbel, more

Journal of Structural Biology > 2009 > 166 > 1 > 103-106

In this technical note we report a tannic acid-mediated osmium impregnation method that, applied after freeze-substitution, increases membrane contrast in cells for transmission electron microscopy and tomography studies. The general staining that is achieved allows visualization of organelles, plasma membrane and associated specializations (e.g. caveolae) in non-post-stained plastic sections by conventional...

article

Effective lead selection for improved protein production in Aspergillus niger based on integrated genomics

Denise I. Jacobs, Maurien M.A. Olsthoorn, Isabelle Maillet, Michiel Akeroyd, more

Fungal Genetics and Biology > 2009 > 46 > 1 Supplement > S141-S152

The filamentous fungus Aspergillus niger is widely exploited for industrial production of enzymes and organic acids. An integrated genomics approach was developed to determine cellular responses of A. niger to protein production in well-controlled fermentations. Different protein extraction methods in combination with automated sample processing and protein identification allowed quantitative analysis...

article

Integrated fluorescence and transmission electron microscopy

Alexandra V. Agronskaia, Jack A. Valentijn, Linda F. van Driel, Chris T.W.M. Schneijdenberg, more

Journal of Structural Biology > 2008 > 164 > 2 > 183-189

Correlative microscopy is a powerful technique that combines the strengths of fluorescence microscopy and electron microscopy. The first enables rapid searching for regions of interest in large fields of view while the latter exhibits superior resolution over a narrow field of view. Routine use of correlative microscopy is seriously hampered by the cumbersome and elaborate experimental procedures...

article

Combined structural and chemical analysis of the anammoxosome: A membrane-bounded intracytoplasmic compartment in anammox bacteria

Laura van Niftrik, Willie J.C. Geerts, Elly G. van Donselaar, Bruno M. Humbel, more

Journal of Structural Biology > 2008 > 161 > 3 > 401-410

Anammox bacteria have unique intracellular membranes that divide their cytoplasm into three separate compartments. The largest and innermost cytoplasmic compartment, the anammoxosome, is hypothesized to be the locus of all catabolic reactions in the anammox metabolism. Electron tomography showed that the anammoxosome and its membrane were highly folded. This finding was confirmed by a transmission...

article

Enrichment of perforate septal pore caps from the basidiomycetous fungus Rhizoctonia solani by combined use of French press, isopycnic centrifugation, and Triton X-100

Kenneth G.A. van Driel, Arend F. van Peer, Han A.B. Wösten, Arie J. Verkleij, more

Journal of Microbiological Methods > 2007 > 71 > 3 > 298-304

Septal pore caps occur in many filamentous basidiomycetes located at both sides of the dolipore septum and are at their base connected to the endoplasmic reticulum. The septal pore cap ultrastructure has been described extensively by the use of electron microscopy, but its composition and function are not yet known. To enable biochemical and functional analyses in the future, we here describe an enrichment...

article

Human embryonic stem cell-derived cardiomyocytes survive and mature in the mouse heart and transiently improve function after myocardial infarction

Linda W. van Laake, Robert Passier, Jantine Monshouwer-Kloots, Arie J. Verkleij, more

Stem Cell Research > 2007 > 1 > 1 > 9-24

Regeneration of the myocardium by transplantation of cardiomyocytes is an emerging therapeutic strategy. Human embryonic stem cells (HESC) form cardiomyocytes readily but until recently at low efficiency, so that preclinical studies on transplantation in animals are only just beginning. Here, we show the results of the first long-term (12 weeks) analysis of the fate of HESC-derived cardiomyocytes...

article

Measuring Location, Size, Distribution, and Loading of NiO Crystallites in Individual SBA-15 Pores by Electron Tomography

Heiner Friedrich, Jelle R. A. Sietsma, Petra E. de Jongh, Arie J. Verkleij, more

Journal of the American Chemical Society > 2007 > 129 > 33 > 10249 - 10254

article

Laser microdissection of fungal septa as visualised by scanning electron microscopy

Kenneth G.A. van Driel, Teun Boekhout, Han A.B. Wösten, Arie J. Verkleij, more

Fungal Genetics and Biology > 2007 > 44 > 6 > 466-473

Laser microdissection has been proven a successful technique to isolate single cells or groups of cells from animal and plant tissue. Here, we demonstrate that laser microdissection is suitable to isolate subcellular parts of fungal hyphae. Dolipore septa of Rhizoctonia solani containing septal pore caps were cut by laser microdissection from sections of mycelium and collected by laser pressure catapulting...

article

Template matching as a tool for annotation of tomograms of stained biological structures

Misjaël N. Lebbink, Willie J.C. Geerts, Theo P. van der Krift, Maurice Bouwhuis, more

Journal of Structural Biology > 2007 > 158 > 3 > 327-335

In recent years, electron tomography has improved our three-dimensional (3D) insight in the structural architecture of cells and organelles. For studies that involve the 3D imaging of stained sections, manual annotation of tomographic data has been an important method to help understand the overall 3D morphology of cellular compartments. Here, we postulate that template matching can provide a tool...

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Search results for: Arie J. Verkleij

Optimizing immuno-labeling for correlative fluorescence and electron microscopy on a single specimen

A biparatopic anti‐EGFR nanobody efficiently inhibits solid tumour growth

Arabidopsis PLETHORA Transcription Factors Control Phyllotaxis

VIS2FIX: A High‐Speed Fixation Method for Immuno‐Electron Microscopy

The making of frozen-hydrated, vitreous lamellas from cells for cryo-electron microscopy

Spiral Coating of the Endothelial Caveolar Membranes as Revealed by Electron Tomography and Template Matching

Sol−Gel Transitions and Liquid Crystal Phase Transitions in Concentrated Aqueous Suspensions of Colloidal Gibbsite Platelets

Quantitative Structural Analysis of Binary Nanocrystal Superlattices by Electron Tomography

Electron Tomography for Heterogeneous Catalysts and Related Nanostructured Materials

Septal pore complex morphology in the Agaricomycotina (Basidiomycota) with emphasis on the Cantharellales and Hymenochaetales

Induced membrane domains as visualized by electron tomography and template matching

Tannic acid-mediated osmium impregnation after freeze-substitution: A strategy to enhance membrane contrast for electron tomography

Effective lead selection for improved protein production in Aspergillus niger based on integrated genomics

Integrated fluorescence and transmission electron microscopy

Combined structural and chemical analysis of the anammoxosome: A membrane-bounded intracytoplasmic compartment in anammox bacteria

Enrichment of perforate septal pore caps from the basidiomycetous fungus Rhizoctonia solani by combined use of French press, isopycnic centrifugation, and Triton X-100

Human embryonic stem cell-derived cardiomyocytes survive and mature in the mouse heart and transiently improve function after myocardial infarction

Measuring Location, Size, Distribution, and Loading of NiO Crystallites in Individual SBA-15 Pores by Electron Tomography

Laser microdissection of fungal septa as visualised by scanning electron microscopy

Template matching as a tool for annotation of tomograms of stained biological structures

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