Search results for: Robert R. Rando

The synthesis of 11-fluoro-all-trans-retinol (11-F-tROL), which is shown to be an excellent substrate for processing by visual cycle enzymes, is described. It is isomerized to its 11-cis congener subsequent to its esterification by lecithin retinol acyl transferase (LRAT) approximately as well as is vitamin A itself. The enzymatic turnover of 11-F-tROL is unaccompanied by enzyme inhibition. The previously...

article

A Palmitoylation Switch Mechanism in the Regulation of the Visual Cycle

Linlong Xue, Deviprasad R. Gollapalli, Pranab Maiti, Wan Jin Jahng, more

Cell > 2004 > 117 > 6 > 761-771

RPE65 is essential for the biosynthesis of 11-cis-retinal, the chromophore of rhodopsin. Here, we show that the membrane-associated form (mRPE65) is triply palmitoylated and is a chaperone for all-trans-retinyl esters, allowing their entry into the visual cycle for processing into 11-cis-retinal. The soluble form of RPE65 (sRPE65) is not palmitoylated and is a chaperone for vitamin A, rather than...

article

RPE65 Operates in the Vertebrate Visual Cycle by Stereospecifically Binding All- trans -Retinyl Esters,

Deviprasad R. Gollapalli, Pranab Maiti, Robert R. Rando

Biochemistry > 2004 > 43 > 22 > 7226 - 7226

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Roles of Cysteine 161 and Tyrosine 154 in the Lecithin−Retinol Acyltransferase Mechanism ^†

Linlong Xue, Robert R. Rando

Biochemistry > 2004 > 43 > 20 > 6120 - 6126

article

Molecular Logic of 11- cis -Retinoid Biosynthesis in a Cone-Dominated Species ^†

Deviprasad R. Gollapalli, Robert R. Rando

Biochemistry > 2003 > 42 > 50 > 14921 - 14929

article

Lecithin Retinol Acyltransferase Is a Founder Member of a Novel Family of Enzymes ^†

Wan Jin Jahng, Linlong Xue, Robert R. Rando

Biochemistry > 2003 > 42 > 44 > 12805 - 12812

article

RPE65 Operates in the Vertebrate Visual Cycle by Stereospecifically Binding All- trans -Retinyl Esters ^†

Deviprasad R. Gollapalli, Pranab Maiti, Robert R. Rando

Biochemistry > 2003 > 42 > 40 > 11824 - 11830

article

Specific inactivation of isomerohydrolase activity by 11-cis-retinoids

Deviprasad R. Gollapalli, Robert R. Rando

BBA - Proteins and Proteomics > 2003 > 1651 > 1-2 > 93-101

The endergonic trans->cis isomerization of retinoids is an essential element in rhodopsin regeneration in vertebrates. All-trans-retinyl esters, which are generated by lecithin retinol acyltransferase (LRAT), are on the isomerization pathway. The critical isomerohydrolase activity, which catalyzes the trans->cis isomerization/hydrolysis reaction of all-trans-retinyl esters, remains to be identified...

article

Purification and Characterization of a Transmembrane Domain-Deleted Form of Lecithin Retinol Acyltransferase ^†

Dean Bok, Alberto Ruiz, Orna Yaron, Wan Jin Jahng, more

Biochemistry > 2003 > 42 > 20 > 6090 - 6098

article

All- trans -retinyl Esters Are the Substrates for Isomerization in the Vertebrate Visual Cycle ^†

Deviprasad R. Gollapalli, Robert R. Rando

Biochemistry > 2003 > 42 > 19 > 5809 - 5818

article

A Cleavable Affinity Biotinylating Agent Reveals a Retinoid Binding Role for RPE65 ^†

Wan Jin Jahng, Charles David, Nasri Nesnas, Koji Nakanishi, more

Biochemistry > 2003 > 42 > 20 > 6159 - 6168

article

Synthesis of (+),(-)-neamine and their positional isomers as potential antibiotics

Do Hyun Ryu, Choon-Hong Tan, Robert R. Rando

Bioorganic & Medicinal Chemistry Letters > 2003 > 13 > 5 > 901-903

The syntheses of (+)-neamine 1, (-)-neamine ent-1 and their positional isomers 2, 3, ent-2 and ent-3 are reported as potential new scaffolds for novel aminoglycoside antibiotics. These isomers exhibit similar inhibitory activities, as shown using an in vitro translation assay. A simple model is proposed to explain this lack of stereospecific binding to the ribosomal RNA.

article

Decoding region bubble size and aminoglycoside antibiotic binding

Do Hyun Ryu, Robert R. Rando

Bioorganic & Medicinal Chemistry Letters > 2002 > 12 > 16 > 2241-2244

Aminoglycoside antibiotics promiscuously bind to structurally diverse RNA molecules containing internal bubbles and bulges with affinities in the μM range. An interesting exception is found in the human 12S mitochondrial decoding region where aminoglycoside binding, unlike in the case of its bacterial and human cytoplasmic counterparts, is absent. Mutations that reduce the size of the bubble in the...

article

Synthesis of biotinylated retinoids for cross-linking and isolation of retinol binding proteins

Nasri Nesnas, Robert R. Rando, Koji Nakanishi

Tetrahedron > 2002 > 58 > 32 > 6577-6584

The synthesis of (3R)-3-[Boc-Lys(biotinyl)-O]-11-cis-retinol bromoacetate and 3-[Boc-Lys(biotinyl)-O]-all trans-retinol chloroacetate is described. These biotinylated retinoids are instrumental in labeling the retinol binding proteins (RBPs) via a nucleophilic displacement of the haloacetate by a residue in the binding site of the protein. The covalently linked biotin will allow for a facile isolation...

article

Stereospecificity of Aminoglycoside−Ribosomal Interactions ^†

Do Hyun Ryu, Alexander Litovchick, Robert R. Rando

Biochemistry > 2002 > 41 > 33 > 10499 - 10509

article

Lecithin Retinol Acyltransferase Forms Functional Homodimers

Wan Jin Jahng, Eric Cheung, Robert R. Rando

Biochemistry > 2002 > 41 > 23 > 7528 - 7528

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Keywords

AMINOGLYCOSIDES (2)
FLUORESCENCE (2)
ISOMEROHYDROLASE (2)
VISUAL CYCLE (2)
VITAMIN A (2)
11-CIS-RETINOL (1)
11-FLUORO-RETINOL (1)
16S RIBOSOMAL RNA (1)
3-METHYLGLYCERIDE: GLYCERIDE: APLYSIATOXIN (1)
ACYL TRANSFERASE (1)
AFFINITY LABELING (1)
ANTAGONISTS (1)
APOPTOSIS (1)
BIOTIN (1)
BIOTINYLATED RETINOIDS (1)
ENDOPROTEOLYSIS (1)
ENZYME CATALYSIS (1)
ENZYME MECHANISM (1)
FARNESYLCYSTEINE (1)
G PROTEIN (1)
HL-60 CELL (1)
HYBRID (1)
IN VITRO SELECTION (1)
INHIBITOR (1)
ISOMERIZATION (1)
ISOMEROHYDROLASE MECHANISM (1)
ISOPRENYLATED PEPTIDE (1)
ISOPRENYLATION (1)
LECITHIN RETINOL ACYL TRANSFERASE (1)
MEMBRANE (1)
METHYLATION (1)
RAS PROTEIN (1)
RBP (1)
RETINOID (1)
RETINOL BINDING PROTEINS (1)
RNA (1)
RPE65 (1)
SIGNAL TRANSDUCTION (1)
SITE-SPECIFIC MUTAGENESIS (1)
THIOL RADICAL (1)
TUMOR PROMOTER (1)
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Elsevier (23)
ACS (16)

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Biochemistry (15)
FEBS Letters (7)
Bioorganic & Medicinal Chemistry (3)
Tetrahedron (3)
Bioorganic & Medicinal Chemistry Letters (2)
Chemistry & Biology (2)
Analytical Biochemistry (1)
BBA - Proteins and Proteomics (1)
Biochemical and Biophysical Research Communications (1)
Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism (1)
Biotechnology Advances (1)
Cell (1)
Chemical Reviews (1)
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INFONA - science communication portal

Search results for: Robert R. Rando

Small Molecule RPE65 Antagonists Limit the Visual Cycle and Prevent Lipofuscin Formation,

Palmitoyl Transferase Activity of Lecithin Retinol Acyl Transferase ^†

Small Molecule RPE65 Antagonists Limit the Visual Cycle and Prevent Lipofuscin Formation ^†

Specificity of Binding of all- trans -Retinyl Ester to RPE65 ^†

On the mechanism of isomerization of all-trans-retinol esters to 11-cis-retinol in retinal pigment epithelial cells: 11-Fluoro-all-trans-retinol as substrate/inhibitor in the visual cycle

A Palmitoylation Switch Mechanism in the Regulation of the Visual Cycle

RPE65 Operates in the Vertebrate Visual Cycle by Stereospecifically Binding All- trans -Retinyl Esters,

Roles of Cysteine 161 and Tyrosine 154 in the Lecithin−Retinol Acyltransferase Mechanism ^†

Molecular Logic of 11- cis -Retinoid Biosynthesis in a Cone-Dominated Species ^†

Lecithin Retinol Acyltransferase Is a Founder Member of a Novel Family of Enzymes ^†

RPE65 Operates in the Vertebrate Visual Cycle by Stereospecifically Binding All- trans -Retinyl Esters ^†

Specific inactivation of isomerohydrolase activity by 11-cis-retinoids

Purification and Characterization of a Transmembrane Domain-Deleted Form of Lecithin Retinol Acyltransferase ^†

All- trans -retinyl Esters Are the Substrates for Isomerization in the Vertebrate Visual Cycle ^†

A Cleavable Affinity Biotinylating Agent Reveals a Retinoid Binding Role for RPE65 ^†

Synthesis of (+),(-)-neamine and their positional isomers as potential antibiotics

Decoding region bubble size and aminoglycoside antibiotic binding

Synthesis of biotinylated retinoids for cross-linking and isolation of retinol binding proteins

Stereospecificity of Aminoglycoside−Ribosomal Interactions ^†

Lecithin Retinol Acyltransferase Forms Functional Homodimers

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