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We study the problem of advance scheduling of ward admission requests in a public hospital, which affects the usage of critical resources such as operating theaters and hospital beds. Given the stochastic arrivals of patients and uncertain usage of resources, it is often infeasible for the planner to devise a risk‐free schedule to meet these requests without violating resource capacity constraints...
Previous studies indicated that the nucleocapsid (N) protein of infectious bronchitis virus (IBV) interacted with specific sequences in the 3' non-coding region of IBV RNA. In order to identify domains in the N protein that bind to RNA, the whole protein (409 amino acids) and six overlapping fragments were expressed as fusion polypeptides with six histidine-tags. Using gel shift assays, the intact...
The infectious bronchitis virus (IBV) nucleocapsid protein was expressed as a bacterial fusion protein which differed from the native protein only in the addition of six amino terminus histidine residues. Using RNA overlay protein blot assays, the recombinant protein was shown to bind to RNA fragments specific for the positive sense 3′ noncoding end of the IBV genome. At greater concentrations of...
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