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Zn 2+ is an essential transition metal required in trace amounts by all living organisms. However, metal excess is cytotoxic and leads to cell damage. Cells rely on transmembrane transporters, with the assistance of other proteins, to establish and maintain Zn 2+ homeostasis. Metal coordination during transport is key to specific transport and unidirectional translocation without the...
The structural-functional roles of 23 cysteines present in the sheep (Na,K)-ATPase α1 subunit were studied using site directed mutagenesis, expression, and kinetics analysis. Twenty of these cysteines were individually substituted by alanine or serine. Cys452, Cys455 and Cys456 were simultaneously replaced by serine. These substitutions were introduced into an ouabain resistant α1 sheep isoform and...
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