Search results for: Marc S. Lewis

Items from 1 to 12 out of 12 results

article

Improving the thermal, radial, and temporal accuracy of the analytical ultracentrifuge through external references

Rodolfo Ghirlando, Andrea Balbo, Grzegorz Piszczek, Patrick H. Brown, more

Analytical Biochemistry > 2013 > 440 > 1 > 81-95

Sedimentation velocity (SV) is a method based on first principles that provides a precise hydrodynamic characterization of macromolecules in solution. Due to recent improvements in data analysis, the accuracy of experimental SV data emerges as a limiting factor in its interpretation. Our goal was to unravel the sources of experimental error and develop improved calibration procedures. We implemented...

article

Planck-Benzinger Thermal Work Function: Thermodynamic Characterization of the Carboxy-Terminus of p53 Peptide Fragments

Paul W. Chun, Marc S. Lewis

The Protein Journal > 2010 > 29 > 8 > 617-630

The thermodynamic parameters for six p53 carboxy-terminus peptide fragments as determined by analytical ultracentrifugal analysis were compared over the experimental temperature range of 275–310 K to evaluate the Gibbs free energy change as a function of temperature, ΔG ^o(T), from 0 to 400 K using our general linear third-order fitting function, ΔG ^o(T) = α + βT ² + γT ³. Data obtained at the...

article

Grb2 adaptor undergoes conformational change upon dimerization

Caleb B. McDonald, Kenneth L. Seldeen, Brian J. Deegan, Marc S. Lewis, more

Archives of Biochemistry and Biophysics > 2008 > 475 > 1 > 25-35

Grb2 is an adaptor protein that couples activated receptor tyrosine kinases to downstream effector molecules such as Ras and Akt. Despite being a central player in mitogenic signaling and a target for therapeutic intervention, the role of Grb2 oligomerization in cellular signaling is not well understood. Here, using the techniques of size-exclusion chromatography, mass spectrometry, analytical ultra-centrifugation...

article

Unfolding, Aggregation, and Amyloid Formation by the Tetramerization Domain from Mutant p53 Associated with Lung Cancer ^†

Yuichiro Higashimoto, Yuya Asanomi, Satoru Takakusagi, Marc S. Lewis, more

Biochemistry > 2006 > 45 > 6 > 1608 - 1619

article

Gal Repressor−Operator−HU Ternary Complex: Pathway of Repressosome Formation

Siddhartha Roy, Emilios K. Dimitriadis, Sudeshna Kar, Mark Geanacopoulos, more

Biochemistry > 2005 > 44 > 14 > 5373 - 5380

article

Analytical Ultracentrifugation Studies of Translin: Analysis of Protein−DNA Interactions Using a Single-Stranded Fluorogenic Oligonucleotide

S. Paul Lee, Elena Fuior, Marc S. Lewis, Myun K. Han

Biochemistry > 2001 > 40 > 46 > 14081 - 14088

article

Kinetics and Thermodynamics of β2-Microglobulin Binding to the α3 Domain of Major Histocompatibility Complex Class I Heavy Chain ^†

Andrea M. Hebert, Jason Strohmaier, Mary C. Whitman, Trina Chen, more

Biochemistry > 2001 > 40 > 17 > 5233 - 5242

article

Analysis of Transport Experiments Using Pseudo-Absorbance Data

Sambit R. Kar, Jon S. Kingsbury, Marc S. Lewis, Tom M. Laue, more

Analytical Biochemistry > 2000 > 285 > 1 > 135-142

The measurement of the concentration distribution of a macromolecule across a solution column by absorption optics usually requires optical transmission profiles of both the sample solution and the buffer, measured under identical conditions, to calculate the absorbance as the logarithm of the ratio of reference to sample intensity. For transport experiments, however, where the changes in the local...

article

Interaction between an intraluminal loop peptide of the inositol 1,4,5-trisphosphate receptor and the near N-terminal peptide of chromogranin A

Seung Hyun Yoo, Marc S. Lewis

FEBS Letters > 1998 > 427 > 1 > 55-58

The near N-terminal region of chromogranin A (CGA) has been shown to be the secretory vesicle membrane binding region, and tetrameric chromogranin A has been demonstrated to bind four molecules of an intraluminal loop peptide of the inositol 1,4,5-trisphosphate (IP ₃ ) receptor. It was therefore necessary to determine whether the conserved near N-terminal region of CGA interacts with the intraluminal...

article

Structure-function relations of heparin-mimetic sulfated xylan oligosaccharides: inhibition of Human Immunodeficiency Virus-1 infectivity in vitro

Audrey Larack Stone, Derek J Melton, Marc S Lewis

Glycoconjugate Journal > 1998 > 15 > 7 > 697-712

Heparins/heparan sulfates modulate the function of proteins and cell membranes in numerous biological systems including normal and disease processes in humans. Heparin has been used for many years as an anticoagulant, and anticoagulant heparin-mimetics were developed several decades ago by chemical sulfation of non-mammalian polysaccharides, e.g., an antithrombotic sulfated xylan. This pharmaceutical,...

article

Effect of Phosphorylation on Tetramerization of the Tumor Suppressor Protein p53

Kazuyasu Sakaguchi, Hiroshi Sakamoto, Dong Xie, John W. Erickson, more

The Protein Journal > 1997 > 16 > 5 > 553-556

Human tumor suppressor protein p53 is a 393-amino acid phosphoprotein that enhances transcription in response to DNA damage from several genes that regulate cell cycle progression. The tetrameric state of p53 is critical to wild-type function; the p53 tetramerization element is located in the C-terminal region of the protein. This region is phosphorylated at several evolutionarily conserved serines,...

article

pH-dependent interaction of an intraluminal loop of inositol 1,4,5-trisphosphate receptor with chromogranin A

Seung Hyun Yoo, Marc S. Lewis

FEBS Letters > 1994 > 341 > 1 > 28-32

The inositol 1,4,5-trisphosphate (IP ₃ )-sensitive Ca ² ⁺ store role of the secretory vesicles of adrenal medullary chromaffin cells is attributed to the presence of high capacity, low affinity Ca ² ⁺ binding protein chromogranin A. Chromogranin A has recently been shown to interact with the protein component(s) on the intraluminal side of the secretory vesicle membrane...

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