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Using 14 C-radiolabeled formate we have found that the rapid form of oxidized cytochrome oxidase can bind up to 1 mol of formate. Treatment of this formate-ligated enzyme with excess cyanide releases 97% of the radiolabel while reduction of formate-labeled enzyme with NADH + ruthenium releases 80-85% of the radioactivity. These data are most simply interpreted by assuming that formate...
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