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Bovine adrenal chromaffin granule cytochrome (cyt) b 561 is a transmembrane hemoprotein that plays a key role in transporting reducing equivalents from ascorbate to dopamine-β-hydroxylase for catecholamine synthesis. We have developed procedures for expression and purification of functional bovine adrenal cyt b 561 in insect and yeast cell systems. The bovine cyt b 561 coding...
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