The interaction of calcyclin with tropomyosin and tropomyosin. actin was studied with fluorescence titrations and photo-reactive crosslinking experiments. Titrations of pyreneiodoacetamide-labeled tropomyosin alone or with actin showed binding of calcyclin to tropomyosin with μM dissociation constants when Ca 2+ was present. UV irradiation of mixtures of calcyclin and gizzard ββ-tropomyosin...
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