Search results for: Bernd H. A. Rehm

Items from 1 to 20 out of 35 results

article

Diphtheria Toxoid Particles as Q Fever Vaccine

Gayathri Sam, Shuxiong Chen, Karren Plain, Ian Marsh, more

Advanced Functional Materials > 34 > 6 > n/a - n/a

There is an unmet need for a stable and nonreactogenic vaccine against the bioterrorism agent, Coxiella burnetii, causing Q fever. Here a safe, effective, and non‐reactogenic Q fever vaccine is developed by employing self‐assembled particles (CPs) composed of cross‐reacting material 197, a nontoxic variant of diphtheria toxin. CPs are designed that incorporate selected C. burnetii antigens and assemble...

article

Assembly of Immunogenic Protein Particles toward Advanced Synthetic Vaccines

Shuxiong Chen, Diana H. Quan, Gayathri Sam, Victoria Ozberk, more

Small > 19 > 8 > n/a - n/a

Immunogenic carrier proteins such as the non‐toxic diphtheria toxin variant, cross‐reacting material 197 (CRM197), are widely used in subunit vaccine formulations to boost immunogenicity of chemically conjugated antigens. Conjugate vaccines are inherently expensive due to laborious manufacturing steps. Here, this work develops a particulate vaccine platform based on using engineered Escherichia coli...

article

Mannuronic Acid in Low‐Risk and Intermediate‐1‐Risk Myelodysplastic Syndromes

Afshin Ghaderi, Sayyed Reza Safaee Nodehi, Tahereh Bakhtiari, Mona Aslani, more

The Journal of Clinical Pharmacology > 60 > 7 > 879 - 888

The discovery of hematologic improvement and bone marrow modification by the drug β‐D mannuronic acid (M2000) during treatment of rheumatoid arthritis in phase 1/2/3 clinical trials prompted us to design a new trial to target hematologic deficits in myelodysplastic syndromes (MDS). In this open‐label, randomized phase 2 clinical trial, the potential effect and tolerability of drug M2000 was assessed...

article

Special Issue on the International Symposium on Biological Polymers 2018

Bernd H. A. Rehm, George Guo‐Qiang Chen

Biotechnology Journal > 14 > 12 > n/a - n/a

article

Alginate Encapsulation of Bioengineered Protein‐Coated Polyhydroxybutyrate Particles: A New Platform for Multifunctional Composite Materials

Kampachiro Ogura, Bernd H. A. Rehm

Advanced Functional Materials > 29 > 37 > n/a - n/a

Immobilization and display of proteins is extensively used to enhance stability and performance of proteins for technical uses such as in biotechnology. Here, self‐assembled nonporous polyhydroxybutyrate (PHB) particles bioengineered to display proteins of interest are subjected to alginate encapsulation processes. The novel composite spheres are fabricated using ionotropic gelation methods. The immunoglobulin...

article

Self‐Assembly: Alginate Encapsulation of Bioengineered Protein‐Coated Polyhydroxybutyrate Particles: A New Platform for Multifunctional Composite Materials (Adv. Funct. Mater. 37/2019)

Kampachiro Ogura, Bernd H. A. Rehm

Advanced Functional Materials > 29 > 37 > n/a - n/a

In article number 1901893, Bernd H. A. Rehm and Kampachiro Ogura engineer bacterial cell factories to assemble nano‐sized polyester particles coated with proteins of interest. These functionalized polyester particles are stable and show promising performance as bioseparation resins and enzyme carriers. The functionality of these non‐porous protein‐coated polyester beads is strongly enhanced by controlled...

article

A randomized, controlled, phase II clinical trial of β‐D‐mannuronic acid (M2000) in pre‐surgical breast cancer patients at early stage (T1‐T2)

Sarvenaz Kashefi, Ramesh Omranipour, Habibollah Mahmoodzadeh, Hamid Ahmadi, more

Clinical and Experimental Pharmacology and Physiology > 46 > 6 > 527 - 532

Following the potent efficacy of β‐D‐Mannuronic acid in a breast cancer murine model, we evaluated the efficacy of this novel non‐steroidal anti‐inflammatory drug in breast cancer patients in the present clinical trial. The study was an 8‐week randomized, controlled, phase II clinical trial (IRCT: 2017012213739N7 (in 48 pre‐surgical breast cancer patients. Patients who had breast cancer at early stage,...

article

International multicenter randomized, placebo-controlled phase III clinical trial of β-d-mannuronic acid in rheumatoid arthritis patients

Zahra Rezaieyazdi, Abid Farooqi, Hossein Soleymani-Salehabadi, Arman Ahmadzadeh, more

Inflammopharmacology > 2019 > 27 > 5 > 911-921

Background The oral administration of drug β-d-mannuronic acid (M2000) showed a potent therapeutic effect in phase I/II study in rheumatoid arthritis (RA) patients. Here, our aim is to assess the efficacy and safety of this new drug in RA patients under a multinational, randomized placebo-controlled phase III clinical trial. Method Patients (n = 288) with active disease at baseline and inadequate...

article

A phase I/II randomized, controlled, clinical trial for assessment of the efficacy and safety of β-d-mannuronic acid in rheumatoid arthritis patients

Hossein Ahmadi, Ahmad Reza Jamshidi, Farhad Gharibdoost, Mahdi Mahmoudi, more

Inflammopharmacology > 2018 > 26 > 3 > 737-745

Background Following the potent efficacy of β-d-mannuronic acid (M2000) in phase I/II trial in ankylosing spondylitis patients, the present clinical trial was conducted to evaluate the efficacy, safety, and tolerability of this novel drug in rheumatoid arthritis (RA) patients who had inadequate response to conventional therapy. Method The study was a 12-week randomized, controlled, phase I/II clinical...

article

Engineering Bacillus megaterium for production of functional intracellular materials

Katrin Grage, Paul McDermott, Bernd H. A. Rehm

Microbial Cell Factories > 2017 > 16 > 1 > 1-12

Background Over the last 10–15 years, a technology has been developed to engineer bacterial poly(3-hydroxybutyrate) (PHB) inclusions as functionalized beads, for applications such as vaccines, diagnostics and enzyme immobilization. This has been achieved by translational fusion of foreign proteins to the PHB synthase (PhaC). The respective fusion protein mediates self-assembly of PHB inclusions displaying...

article

Purification of therapeutic proteins mediated by in vivo polyester immobilized sortase

Jinping Du, Bernd H. A. Rehm

Biotechnology Letters > 2018 > 40 > 2 > 369-373

Objectives To overcome laborious and costly procedures often associated with therapeutic protein production and purification, in vivo polyester immobilized sortase is explored for the production of human tumor necrosis factor alpha (TNFα) and human interferon alpha 2b (IFNα2b) by Escherichia coli. Results Hybrid genes encoding PhaC-Sortase-TNFα or PhaC-Sortase-IFNα2b fusions (with a LPETG recognition...

article

Purification of target proteins from intracellular inclusions mediated by intein cleavable polyhydroxyalkanoate synthase fusions

Jinping Du, Bernd H. A. Rehm

Microbial Cell Factories > 2017 > 16 > 1 > 1-13

Background Recombinant protein production and purification from Escherichia coli is often accompanied with expensive and complicated procedures, especially for therapeutic proteins. Here it was demonstrated that, by using an intein cleavable polyhydroxyalkanoate synthase fusion, recombinant proteins can be first produced and sequestered on a natural resin, the polyhydroxyalkanoate (PHA) inclusions,...

article

The effects of β-d-mannuronic acid (M2000), as a novel NSAID, on COX1 and COX2 activities and gene expression in ankylosing spondylitis patients and the murine monocyte/macrophage, J774 cell line

Fahimeh Jafarnezhad-Ansariha, Mir Saeed Yekaninejad, Ahmad-reza Jamshidi, Reza Mansouri, more

Inflammopharmacology > 2018 > 26 > 2 > 375-384

Ankylosing spondylitis (AS) is a debilitating chronic inflammatory disease with genetic predisposition, which is characterized by the involvement of spine and sacroiliac joints. Due to the relatively unsuccessful treatments, we designed β-d-mannuronic (M2000) with the beneficial effects in various experimental models as a novel non-steroidal anti-inflammatory drug (NSAID). The aims of our present...

article

Assessment of farm soil, biochar, compost and weathered pine mulch to mitigate methane emissions

Rashad Syed, Surinder Saggar, Kevin Tate, Bernd H. A. Rehm

Applied Microbiology and Biotechnology > 2016 > 100 > 21 > 9365-9379

Previous studies have demonstrated the effective utility of volcanic pumice soil to mitigate both high and low levels of methane (CH₄) emissions through the activity of both γ-proteobacterial (type I) and α-proteobacterial (type II) aerobic methanotrophs. However, the limited availability of volcanic pumice soil necessitates the assessment of other farm soils and potentially suitable, economical and...

article

Does acidification of a soil biofilter compromise its methane-oxidising capacity?

Rashad Syed, Surinder Saggar, Kevin Tate, Bernd H. A. Rehm

Biology and Fertility of Soils > 2016 > 52 > 4 > 573-583

A biofilter made using volcanic pumice soil from a landfill in Taupo, New Zealand has been found to mitigate CH₄ emissions from New Zealand dairy effluent ponds. However, the biofilter after drying out following almost 5 years of use removed little or no CH₄. Furthermore, H₂S present in the biogas (from the dairy effluent ponds) had increased the acidity (pH) in the soil biofilter from 5.2 to 3.72...

chapter

Microbial Synthesis of Biodegradable Polyesters: Processes, Products, Applications

Bernd H. A. Rehm

Biodegradable Polyesters > 47 - 72

This chapter discusses the present literature on bacterial polyhydroxyalkanoate (PHA) synthesis, PHA granules, their biogenesis, and structure, and on protein engineering approaches of associated proteins aiming at the design of PHA granules as biobeads for biomedical use. The biologically produced biopolyesters comprise a complex class of polyoxoesters. The nucleotide sequences of 88 PHA synthase...

article

Membrane-anchored MucR mediates nitrate-dependent regulation of alginate production in Pseudomonas aeruginosa

Yajie Wang, Iain D. Hay, Zahid U. Rehman, Bernd H. A. Rehm

Applied Microbiology and Biotechnology > 2015 > 99 > 17 > 7253-7265

Alginates exhibit unique material properties suitable for medical and industrial applications. However, if produced by Pseudomonas aeruginosa, it is an important virulence factor in infection of cystic fibrosis patients. The alginate biosynthesis machinery is activated by c-di-GMP imparted by the inner membrane protein, MucR. Here, it was shown that MucR impairs alginate production in response to...

article

Insights into the surface topology of polyhydroxyalkanoate synthase: self-assembly of functionalized inclusions

David O. Hooks, Bernd H. A. Rehm

Applied Microbiology and Biotechnology > 2015 > 99 > 19 > 8045-8053

The polyhydroxyalkanoate (PHA) synthase catalyzes the synthesis of PHA and remains attached to the hydrophobic PHA inclusions it creates. Although this feature is actively exploited to generate functionalized biobeads via protein engineering, little is known about the structure of the PHA synthase. Here, the surface topology of Ralstonia eutropha PHA synthase was probed to inform rational protein...

article

Immobilization of active lipase B from Candida antarctica on the surface of polyhydroxyalkanoate inclusions

Anika C. Jahns, Bernd H. A. Rehm

Biotechnology Letters > 2015 > 37 > 4 > 831-835

Polyhydroxyalkanoate (PHA) beads, recombinantly produced in Escherichia coli , were functionalized to display lipase B from Candida antarctica as translational protein fusion. The respective beads were characterized in respect to protein content, functionality, long term storage capacity and re-usability. The direct fusion of the PHA synthase, PhaC, to lipase B yielded...

article

Surface display of highly-stable Desulfovibrio vulgaris carbonic anhydrase on polyester beads for CO₂ capture

David O. Hooks, Bernd H. A. Rehm

Biotechnology Letters > 2015 > 37 > 7 > 1415-1420

Objectives To engineer a recombinant Escherichia coli to produce polyhydroxyalkanoate biopolymer beads displaying carbonic anhydrase (CA) from Desulfovibrio vulgaris str. “Miyazaki F” (DvCA). Results The highest measured specific activity of the immobilised CA was 211 U/mg DvCA. The immobilised CA was thermostable, retaining 114 U/mg DvCA of activity after incubation at 90 °C for 1 h. Additionally,...

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ACINETOBACTER CALCOACETICUSAZOTOBACTER VINELANDIIPSEUDOMONAS PUTIDARALSTONIA EUTROPHARHODOCOCCUS OPACUS NILE RED NILE BLUE A VIABLE COLONY STAINING POLYHYDROXYALKANOIC ACID TRIACYLGLYCEROLS LIPID INCLUSIONS POLY(3-HYDROXYBUTYRIC ACID) POLY(4-HYDROXYBUTYRIC ACID) POLY(3-HYDROXYOCTANOIC ACID) (1)
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INFONA - science communication portal

Search results for: Bernd H. A. Rehm

Diphtheria Toxoid Particles as Q Fever Vaccine

Assembly of Immunogenic Protein Particles toward Advanced Synthetic Vaccines

Mannuronic Acid in Low‐Risk and Intermediate‐1‐Risk Myelodysplastic Syndromes

Special Issue on the International Symposium on Biological Polymers 2018

Alginate Encapsulation of Bioengineered Protein‐Coated Polyhydroxybutyrate Particles: A New Platform for Multifunctional Composite Materials

Self‐Assembly: Alginate Encapsulation of Bioengineered Protein‐Coated Polyhydroxybutyrate Particles: A New Platform for Multifunctional Composite Materials (Adv. Funct. Mater. 37/2019)

A randomized, controlled, phase II clinical trial of β‐D‐mannuronic acid (M2000) in pre‐surgical breast cancer patients at early stage (T1‐T2)

International multicenter randomized, placebo-controlled phase III clinical trial of β-d-mannuronic acid in rheumatoid arthritis patients

A phase I/II randomized, controlled, clinical trial for assessment of the efficacy and safety of β-d-mannuronic acid in rheumatoid arthritis patients

Engineering Bacillus megaterium for production of functional intracellular materials

Purification of therapeutic proteins mediated by in vivo polyester immobilized sortase

Purification of target proteins from intracellular inclusions mediated by intein cleavable polyhydroxyalkanoate synthase fusions

The effects of β-d-mannuronic acid (M2000), as a novel NSAID, on COX1 and COX2 activities and gene expression in ankylosing spondylitis patients and the murine monocyte/macrophage, J774 cell line

Assessment of farm soil, biochar, compost and weathered pine mulch to mitigate methane emissions

Does acidification of a soil biofilter compromise its methane-oxidising capacity?

Microbial Synthesis of Biodegradable Polyesters: Processes, Products, Applications

Membrane-anchored MucR mediates nitrate-dependent regulation of alginate production in Pseudomonas aeruginosa

Insights into the surface topology of polyhydroxyalkanoate synthase: self-assembly of functionalized inclusions

Immobilization of active lipase B from Candida antarctica on the surface of polyhydroxyalkanoate inclusions

Surface display of highly-stable Desulfovibrio vulgaris carbonic anhydrase on polyester beads for CO₂ capture

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